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1529-240137412017Oct11The Journal of neuroscience : the official journal of the Society for NeuroscienceJ NeurosciMAP1B Light Chain Modulates Synaptic Transmission via AMPA Receptor Intracellular Trapping.994599639945-996310.1523/JNEUROSCI.0505-17.2017The regulated transport of AMPA-type glutamate receptors (AMPARs) to the synaptic membrane is a key mechanism to determine the strength of excitatory synaptic transmission in the brain. In this work, we uncovered a new role for the microtubule-associated protein MAP1B in modulating access of AMPARs to the postsynaptic membrane. Using mice and rats of either sex, we show that MAP1B light chain (LC) accumulates in the somatodendritic compartment of hippocampal neurons, where it forms immobile complexes on microtubules that limit vesicular transport. These complexes restrict AMPAR dendritic mobility, leading to the intracellular trapping of receptors and impairing their access to the dendritic surface and spines. Accordingly, increasing MAP1B-LC expression depresses AMPAR-mediated synaptic transmission. This effect is specific for the GluA2 subunit of the AMPAR and requires glutamate receptor interacting protein 1 (GRIP1) interaction with MAP1B-LC. Therefore, MAP1B-LC represents an alternative link between GRIP1-AMPARs and microtubules that does not result in productive transport, but rather limits AMPAR availability for synaptic insertion, with a direct impact on synaptic transmission.SIGNIFICANCE STATEMENT The ability of neurons to modify their synaptic connections, known as synaptic plasticity, is accepted as the cellular basis for learning and memory. One mechanism for synaptic plasticity is the regulated addition and removal of AMPA-type glutamate receptors (AMPARs) at excitatory synapses. In this study, we found that a microtubule-associated protein, MAP1B light chain (MAP1B-LC), participates in this process. MAP1B-LC forms immobile complexes along dendrites. These complexes limit intracellular vesicular trafficking and trap AMPARs inside the dendritic shaft. In this manner, MAP1B restricts the access of AMPARs to dendritic spines and the postsynaptic membrane, contributing to downregulating synaptic transmission.Copyright © 2017 the authors 0270-6474/17/379945-19$15.00/0.PalenzuelaRocíoRDepartment of Neurobiology, Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid 28049, Spain, and.School of Experimental Sciences, Universidad Francisco de Vitoria, Pozuelo de Alarcón, Madrid 28223, Spain.GutiérrezYolandaYDepartment of Neurobiology, Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid 28049, Spain, and.DraffinJonathan EJEDepartment of Neurobiology, Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid 28049, Spain, and.LarioArgentinaADepartment of Neurobiology, Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid 28049, Spain, and.BenoistMarionMDepartment of Neurobiology, Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid 28049, Spain, and.EstebanJosé AJA0000-0002-3759-3300Department of Neurobiology, Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid), Madrid 28049, Spain, and jaesteban@cbm.csic.es.engU24 NS050606NSNINDS NIH HHSUnited StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't20170913
United StatesJ Neurosci81021400270-64740Adaptor Proteins, Signal Transducing0Microtubule-Associated Proteins0Nerve Tissue Proteins0Receptors, AMPA0Grip1 protein, mouse0microtubule-associated protein 1BP6W5IXV8V9glutamate receptor ionotropic, AMPA 2IMAdaptor Proteins, Signal TransducingmetabolismAnimalsDendritesdrug effectsDendritic SpinesphysiologyFemaleHippocampuscytologymetabolismMaleMiceMicrotubule-Associated ProteinsbiosynthesisgeneticsphysiologyNerve Tissue ProteinsmetabolismNeuronsmetabolismRatsRats, WistarReceptors, AMPAmetabolismphysiologySynapsesphysiologySynaptic TransmissiongeneticsGRIP1GluA1GluA2cytoskeletonhippocampusspine20172222017817201781820179156020171024602017915602018411ppublish28904092PMC659659510.1523/JNEUROSCI.0505-17.2017JNEUROSCI.0505-17.2017Alberi S, Boda B, Steiner P, Nikonenko I, Hirling H, Muller D (2005) The endosomal protein NEEP21 regulates AMPA receptor-mediated synaptic transmission and plasticity in the hippocampus. 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