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1878-15511812010Jan19Developmental cellDev CelldFezf/Earmuff maintains the restricted developmental potential of intermediate neural progenitors in Drosophila.126135126-3510.1016/j.devcel.2009.12.007To ensure normal development and maintenance of homeostasis, the extensive developmental potential of stem cells must be functionally distinguished from the limited developmental potential of transit amplifying cells. Yet the mechanisms that restrict the developmental potential of transit amplifying cells are poorly understood. Here we show that the evolutionarily conserved transcription factor dFezf/Earmuff (Erm) functions cell-autonomously to maintain the restricted developmental potential of the intermediate neural progenitors generated by type II neuroblasts in Drosophila larval brains. Although erm mutant intermediate neural progenitors are correctly specified and show normal apical-basal cortical polarity, they can dedifferentiate back into a neuroblast state, functionally indistinguishable from normal type II neuroblasts. Erm restricts the potential of intermediate neural progenitors by activating Prospero to limit proliferation and by antagonizing Notch signaling to prevent dedifferentiation. We conclude that Erm dependence functionally distinguishes intermediate neural progenitors from neuroblasts in the Drosophila larval brain, balancing neurogenesis with stem cell maintenance.(c) 2010 Elsevier Inc. All rights reserved.WengMoMDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA.GoldenKrista LKLLeeCheng-YuCYengR01 GM092818GMNIGMS NIH HHSUnited StatesJournal ArticleResearch Support, Non-U.S. Gov't
United StatesDev Cell1011200281534-58070Carrier Proteins0Drosophila Proteins0N protein, Drosophila0Nerve Tissue Proteins0Nuclear Proteins0Receptors, Notch0Transcription Factors0Zebrafish Proteins0erm protein, Drosophila0fezf2 protein, zebrafish0pros protein, DrosophilaIMAnimalsCarrier ProteinsgeneticsmetabolismCell DifferentiationphysiologyCell LineagephysiologyCell PolarityphysiologyCell ProliferationDrosophilacytologyembryologyDrosophila ProteinsgeneticsmetabolismGene Expression Regulation, DevelopmentalphysiologyLarvacytologygrowth & developmentNerve Tissue ProteinsgeneticsmetabolismNervous SystemcytologyembryologyNeurogenesisphysiologyNeuronscytologymetabolismNuclear ProteinsgeneticsmetabolismReceptors, NotchgeneticsmetabolismStem CellscytologymetabolismTranscription FactorsgeneticsmetabolismZebrafish ProteinsgeneticsmetabolismZinc Fingersgenetics2009327200982120091272010216602010216602010320602019819ppublish20152183NIHMS877127PMC669951410.1016/j.devcel.2009.12.007S1534-5807(09)00524-3Bello BC, Izergina N, Caussinus E, and Reichert H (2008). 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