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1529-240128222008May28The Journal of neuroscience : the official journal of the Society for NeuroscienceJ NeurosciThe glutamate receptor-interacting protein family of GluR2-binding proteins is required for long-term synaptic depression expression in cerebellar Purkinje cells.575257555752-510.1523/JNEUROSCI.0654-08.2008Glutamate receptor-interacting protein 1 (GRIP1) and GRIP2 are closely related proteins that bind GluR2-containing AMPA receptors and couple them to structural and signaling complexes in neurons. Cerebellar long-term synaptic depression (LTD) is a model system of synaptic plasticity that is expressed by persistent internalization of GluR2-containing AMPA receptors. Here, we show that genetic deletion of both GRIP1 and GRIP2 blocks LTD expression in primary cultures of mouse cerebellar neurons but that single deletion of either isoform allows LTD to occur. In GRIP1/2 double knock-out Purkinje cells, LTD can be fully rescued by a plasmid-driving expression of GRIP1 and partially rescued by a GRIP2 plasmid. These results indicate that the GRIP family comprises an essential molecular component for cerebellar LTD.TakamiyaKogoKDepartment of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.MaoLifangLHuganirRichard LRLLindenDavid JDJengR01 NS036715NSNINDS NIH HHSUnited StatesR37 MH051106MHNIMH NIH HHSUnited StatesP50 MH068830MHNIMH NIH HHSUnited StatesHHMIHoward Hughes Medical InstituteUnited StatesR37 MH51106MHNIMH NIH HHSUnited StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't
United StatesJ Neurosci81021400270-64740Adaptor Proteins, Signal Transducing0Carrier Proteins0Excitatory Amino Acid Agonists0Grip1 protein, mouse0Grip2 protein, mouse0Intracellular Signaling Peptides and Proteins0Nerve Tissue Proteins0enhanced green fluorescent protein147336-22-9Green Fluorescent Proteins3KX376GY7LGlutamic Acid534-82-7MethoxyhydroxyphenylglycolUEH9K539KJ3,4-dihydroxyphenylglycolIMAdaptor Proteins, Signal TransducingdeficiencyphysiologyAnimalsCarrier ProteinsphysiologyCells, CulturedCerebellumcytologyElectric StimulationmethodsEmbryo, MammalianExcitatory Amino Acid AgonistspharmacologyGlutamic AcidpharmacologyGreen Fluorescent ProteinsbiosynthesisgeneticsIntracellular Signaling Peptides and ProteinsLong-Term Synaptic Depressiondrug effectsphysiologyradiation effectsMethoxyhydroxyphenylglycolanalogs & derivativespharmacologyMiceMice, Inbred C57BLMice, TransgenicMutationphysiologyNerve Tissue ProteinsdeficiencyphysiologyPDZ DomainsphysiologyPatch-Clamp TechniquesmethodsPurkinje Cellsphysiology2008530902008729020085309020081128ppublish18509036NIHMS56925PMC258708310.1523/JNEUROSCI.0654-08.200828/22/5752Bladt F, Tafuri A, Gelkop S, Langille L, Pawson T. 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