333197952021052020211204
2045-23221012020Dec15Scientific reportsSci RepIdentification of potential transcription factors that enhance human iPSC generation.21950219502195010.1038/s41598-020-78932-9Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platform we have identified and validated the differential gene expression of five transcription factors (TFs) (GBX2, NANOGP8, SP8, PEG3, and ZIC1) that were associated with a remarkable increase in the number of iPSC colonies generated from a patient with Parkinson's disease. We have applied different bioinformatics tools (Gene ontology, protein-protein interaction, and signaling pathways analyses) to investigate the possible roles of these TFs in pluripotency and developmental process. Interestingly, GBX2, NANOGP8, SP8, PEG3, and ZIC1 were found to play a role in maintaining pluripotency, regulating self-renewal stages, and interacting with other factors that are involved in pluripotency regulation including OCT4, SOX2, NANOG, and KLF4. Therefore, the TFs identified in this study could be used as additional transcription factors that enhance reprogramming efficiency to boost iPSC generation technology.SwaidanNuha TNTNeurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar.Salloum-AsfarSalamSNeurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar.PalangiFreshtehFNeurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar.ErrafiiKhaoulaKGenomics Core Facility, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation, Doha, Qatar.College of Health and Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha, Qatar.SolimanNada HNHBasic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar.AboughaliaAhmed TATBasic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar.WaliAbdul Haseeb SAHSBasic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar.AbdullaSara ASANeurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar. saabdulla@hbku.edu.qa.EmaraMohamed MMMBasic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar. memara@qu.edu.qa.Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha, Qatar. memara@qu.edu.qa.engJournal ArticleResearch Support, Non-U.S. Gov't20201215
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