eLife

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2050-084X72018Mar07eLifeElifeDrosophila Fezf coordinates laminar-specific connectivity through cell-intrinsic and cell-extrinsic mechanisms.e3396210.7554/eLife.33962Laminar arrangement of neural connections is a fundamental feature of neural circuit organization. Identifying mechanisms that coordinate neural connections within correct layers is thus vital for understanding how neural circuits are assembled. In the medulla of the Drosophila visual system neurons form connections within ten parallel layers. The M3 layer receives input from two neuron types that sequentially innervate M3 during development. Here we show that M3-specific innervation by both neurons is coordinated by Drosophila Fezf (dFezf), a conserved transcription factor that is selectively expressed by the earlier targeting input neuron. In this cell, dFezf instructs layer specificity and activates the expression of a secreted molecule (Netrin) that regulates the layer specificity of the other input neuron. We propose that employment of transcriptional modules that cell-intrinsically target neurons to specific layers, and cell-extrinsically recruit other neurons is a general mechanism for building layered networks of neural connections.© 2018, Peng et al.PengJingJDepartment of Neurobiology, Harvard Medical School, Boston, United States.SantiagoIvan JIJDepartment of Neurobiology, Harvard Medical School, Boston, United States.AhnCurieCDepartment of Neurobiology, Harvard Medical School, Boston, United States.GurBurakBEuropean Neuroscience Institute, Göttingen, Germany.TsuiC KimberlyCKDepartment of Genetics, Stanford University, Stanford, United States.SuZhixiaoZDepartment of Neurobiology, Harvard Medical School, Boston, United States.XuChundiCDepartment of Neurobiology, Harvard Medical School, Boston, United States.KarakhanyanAzizADepartment of Neurobiology, Harvard Medical School, Boston, United States.SiliesMarionMEuropean Neuroscience Institute, Göttingen, Germany.PecotMatthew YMY0000-0001-8241-8002Department of Neurobiology, Harvard Medical School, Boston, United States.engK01 NS094545NSNINDS NIH HHSUnited StatesGilliam Fellowship for Advanced StudyHHMIHoward Hughes Medical InstituteUnited StatesJournal ArticleResearch Support, Non-U.S. Gov't20180307

EnglandElife1015796142050-084X0Drosophila Proteins0Netrins0Transcription Factors0erm protein, DrosophilaIMAnimalsDrosophila ProteinsgeneticsDrosophila melanogastergeneticsgrowth & developmentGene Expression Regulation, DevelopmentalMedulla Oblongatagrowth & developmentmetabolismNerve Netgrowth & developmentNetrinsgeneticsNeurogenesisgeneticsNeuronsmetabolismPhotoreceptor Cells, InvertebratemetabolismSynapsesgeneticsTranscription FactorsgeneticsVisual Pathwaysgrowth & developmentD. melanogasterFezflaminar specificityneurosciencetranscription factorsJP, IS, CA, BG, CT, ZS, CX, AK, MS, MP No competing interests declared20171130201836201838602019726020183860201837epublish29513217PMC585446510.7554/eLife.3396233962Akin O, Zipursky SL. Frazzled promotes growth cone attachment at the source of a Netrin gradient in the Drosophila visual system. eLife. 2016;5:e20762. doi: 10.7554/eLife.20762.10.7554/eLife.20762PMC510859227743477Baier H. 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