{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"10197531"},"DateCompleted":{"Year":"1999","Month":"04","Day":"27"},"DateRevised":{"Year":"2022","Month":"02","Day":"24"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0896-6273"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"22","Issue":"3","PubDate":{"Year":"1999","Month":"Mar"}},"Title":"Neuron","ISOAbbreviation":"Neuron"},"ArticleTitle":"EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains.","Pagination":{"StartPage":"511","EndPage":"524","MedlinePgn":"511-24"},"Abstract":{"AbstractText":["Transmembrane ephrinB proteins have important functions during embryonic patterning as ligands for Eph receptor tyrosine kinases and presumably as signal-transducing receptor-like molecules. Consistent with \"reverse\" signaling, ephrinB1 is localized in sphingo-lipid\/cholesterol-enriched raft microdomains, platforms for the localized concentration and activation of signaling molecules. Glutamate receptor-interacting protein (GRIP) and a highly related protein, which we have termed GRIP2, are recruited into these rafts through association with the C-terminal PDZ target site of ephrinB1. Stimulation of ephrinB1 with soluble EphB2 receptor ectodomain causes the formation of large raft patches that also contain GRIP proteins. Moreover, a GRIP-associated serine\/threonine kinase activity is recruited into ephrinB1-GRIP complexes. Our findings suggest that GRIP proteins provide a scaffold for the assembly of a multiprotein signaling complex downstream of ephrinB ligands."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Br\u00fcckner","ForeName":"K","Initials":"K","AffiliationInfo":[{"Affiliation":"Developmental Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Pablo Labrador","ForeName":"J","Initials":"J"},{"@attributes":{"ValidYN":"Y"},"LastName":"Scheiffele","ForeName":"P","Initials":"P"},{"@attributes":{"ValidYN":"Y"},"LastName":"Herb","ForeName":"A","Initials":"A"},{"@attributes":{"ValidYN":"Y"},"LastName":"Seeburg","ForeName":"P H","Initials":"PH"},{"@attributes":{"ValidYN":"Y"},"LastName":"Klein","ForeName":"R","Initials":"R"}]},"Language":["eng"],"DataBankList":{"@attributes":{"CompleteYN":"Y"},"DataBank":[{"DataBankName":"GENBANK","AccessionNumberList":{"AccessionNumber":["AF072509","AJ133439"]}}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Neuron","NlmUniqueID":"8809320","ISSNLinking":"0896-6273"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D048868"},"@text":"Adaptor Proteins, Signal Transducing"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D002352"},"@text":"Carrier Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D036387"},"@text":"Ephrin-B1"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491309"},"@text":"Grip1 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491308"},"@text":"Grip1 protein, rat"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C485567"},"@text":"Grip2 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C118486"},"@text":"Grip2 protein, rat"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D036341"},"@text":"Intercellular Signaling Peptides and Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D047908"},"@text":"Intracellular Signaling Peptides and Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D008024"},"@text":"Ligands"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D008565"},"@text":"Membrane Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D009419"},"@text":"Nerve Tissue Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D019606"},"@text":"Phosphoamino Acids"}},{"RegistryNumber":"EC 2.7.11.1","NameOfSubstance":{"@attributes":{"UI":"D017346"},"@text":"Protein Serine-Threonine Kinases"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D048868","MajorTopicYN":"N"},"@text":"Adaptor Proteins, Signal Transducing"}},{"DescriptorName":{"@attributes":{"UI":"D000595","MajorTopicYN":"N"},"@text":"Amino Acid Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D015153","MajorTopicYN":"N"},"@text":"Blotting, Western"}},{"DescriptorName":{"@attributes":{"UI":"D001921","MajorTopicYN":"N"},"@text":"Brain"},"QualifierName":[{"@attributes":{"UI":"Q000196","MajorTopicYN":"N"},"@text":"embryology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002352","MajorTopicYN":"N"},"@text":"Carrier Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"},{"@attributes":{"UI":"Q000502","MajorTopicYN":"Y"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D002460","MajorTopicYN":"N"},"@text":"Cell Line"}},{"DescriptorName":{"@attributes":{"UI":"D002462","MajorTopicYN":"N"},"@text":"Cell Membrane"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"},{"@attributes":{"UI":"Q000648","MajorTopicYN":"N"},"@text":"ultrastructure"}]},{"DescriptorName":{"@attributes":{"UI":"D036387","MajorTopicYN":"N"},"@text":"Ephrin-B1"}},{"DescriptorName":{"@attributes":{"UI":"D017353","MajorTopicYN":"N"},"@text":"Gene Deletion"}},{"DescriptorName":{"@attributes":{"UI":"D017403","MajorTopicYN":"N"},"@text":"In Situ Hybridization"}},{"DescriptorName":{"@attributes":{"UI":"D036341","MajorTopicYN":"N"},"@text":"Intercellular Signaling Peptides and Proteins"}},{"DescriptorName":{"@attributes":{"UI":"D047908","MajorTopicYN":"N"},"@text":"Intracellular Signaling Peptides and Proteins"}},{"DescriptorName":{"@attributes":{"UI":"D008024","MajorTopicYN":"N"},"@text":"Ligands"}},{"DescriptorName":{"@attributes":{"UI":"D008565","MajorTopicYN":"N"},"@text":"Membrane 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Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"},{"@attributes":{"UI":"Q000502","MajorTopicYN":"Y"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D009474","MajorTopicYN":"N"},"@text":"Neurons"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"},{"@attributes":{"UI":"Q000648","MajorTopicYN":"N"},"@text":"ultrastructure"}]},{"DescriptorName":{"@attributes":{"UI":"D009928","MajorTopicYN":"N"},"@text":"Organ Specificity"}},{"DescriptorName":{"@attributes":{"UI":"D019606","MajorTopicYN":"N"},"@text":"Phosphoamino Acids"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D011233","MajorTopicYN":"N"},"@text":"Precipitin Tests"}},{"DescriptorName":{"@attributes":{"UI":"D017346","MajorTopicYN":"N"},"@text":"Protein Serine-Threonine 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