{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"10678167"},"DateCompleted":{"Year":"2000","Month":"03","Day":"09"},"DateRevised":{"Year":"2019","Month":"09","Day":"15"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"1097-2765"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"5","Issue":"1","PubDate":{"Year":"2000","Month":"Jan"}},"Title":"Molecular cell","ISOAbbreviation":"Mol Cell"},"ArticleTitle":"Left-right asymmetric expression of lefty2 and nodal is induced by a signaling pathway that includes the transcription factor FAST2.","Pagination":{"StartPage":"35","EndPage":"47","MedlinePgn":"35-47"},"Abstract":{"AbstractText":["The left-right (L-R) asymmetric expression of lefty2 and nodal is controlled by a left side-specific enhancer (ASE). The transcription factor FAST2, which can mediate signaling by TGF beta and activin, has now been identified as a protein that binds to a conserved sequence in ASE. These FAST2 binding sites were both essential and sufficient for L-R asymmetric gene expression. The Fast2 gene is bilaterally expressed when nodal and lefty2 are expressed on the left side. TGF beta and activin can activate the ASE activity in a FAST2-dependent manner, while Nodal can do so in the presence of an EGF-CFC protein. These results suggest that the asymmetric expression of lefty2 and nodal is induced by a left side-specific TGF beta-related factor, which is most likely Nodal itself."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Saijoh","ForeName":"Y","Initials":"Y","AffiliationInfo":[{"Affiliation":"Division of Molecular Biology, Osaka University, Japan."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Adachi","ForeName":"H","Initials":"H"},{"@attributes":{"ValidYN":"Y"},"LastName":"Sakuma","ForeName":"R","Initials":"R"},{"@attributes":{"ValidYN":"Y"},"LastName":"Yeo","ForeName":"C Y","Initials":"CY"},{"@attributes":{"ValidYN":"Y"},"LastName":"Yashiro","ForeName":"K","Initials":"K"},{"@attributes":{"ValidYN":"Y"},"LastName":"Watanabe","ForeName":"M","Initials":"M"},{"@attributes":{"ValidYN":"Y"},"LastName":"Hashiguchi","ForeName":"H","Initials":"H"},{"@attributes":{"ValidYN":"Y"},"LastName":"Mochida","ForeName":"K","Initials":"K"},{"@attributes":{"ValidYN":"Y"},"LastName":"Ohishi","ForeName":"S","Initials":"S"},{"@attributes":{"ValidYN":"Y"},"LastName":"Kawabata","ForeName":"M","Initials":"M"},{"@attributes":{"ValidYN":"Y"},"LastName":"Miyazono","ForeName":"K","Initials":"K"},{"@attributes":{"ValidYN":"Y"},"LastName":"Whitman","ForeName":"M","Initials":"M"},{"@attributes":{"ValidYN":"Y"},"LastName":"Hamada","ForeName":"H","Initials":"H"}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Mol Cell","NlmUniqueID":"9802571","ISSNLinking":"1097-2765"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D004268"},"@text":"DNA-Binding Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D051858"},"@text":"Forkhead Transcription Factors"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491117"},"@text":"Foxh1 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D055458"},"@text":"Left-Right Determination Factors"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D055457"},"@text":"Nodal Protein"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C526008"},"@text":"Nodal protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D011993"},"@text":"Recombinant Fusion Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D011994"},"@text":"Recombinant Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D014157"},"@text":"Transcription Factors"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D016212"},"@text":"Transforming Growth Factor beta"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D001483","MajorTopicYN":"N"},"@text":"Base Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D003001","MajorTopicYN":"N"},"@text":"Cloning, Molecular"}},{"DescriptorName":{"@attributes":{"UI":"D017124","MajorTopicYN":"N"},"@text":"Conserved Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D004268","MajorTopicYN":"N"},"@text":"DNA-Binding Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D004622","MajorTopicYN":"N"},"@text":"Embryo, Mammalian"}},{"DescriptorName":{"@attributes":{"UI":"D004625","MajorTopicYN":"N"},"@text":"Embryo, Nonmammalian"}},{"DescriptorName":{"@attributes":{"UI":"D004742","MajorTopicYN":"N"},"@text":"Enhancer Elements, Genetic"}},{"DescriptorName":{"@attributes":{"UI":"D005246","MajorTopicYN":"N"},"@text":"Feedback"}},{"DescriptorName":{"@attributes":{"UI":"D051858","MajorTopicYN":"N"},"@text":"Forkhead Transcription Factors"}},{"DescriptorName":{"@attributes":{"UI":"D005786","MajorTopicYN":"Y"},"@text":"Gene Expression Regulation"}},{"DescriptorName":{"@attributes":{"UI":"D055458","MajorTopicYN":"N"},"@text":"Left-Right Determination Factors"}},{"DescriptorName":{"@attributes":{"UI":"D051379","MajorTopicYN":"N"},"@text":"Mice"}},{"DescriptorName":{"@attributes":{"UI":"D008822","MajorTopicYN":"N"},"@text":"Mice, Transgenic"}},{"DescriptorName":{"@attributes":{"UI":"D055457","MajorTopicYN":"N"},"@text":"Nodal Protein"}},{"DescriptorName":{"@attributes":{"UI":"D011993","MajorTopicYN":"N"},"@text":"Recombinant Fusion Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000096","MajorTopicYN":"N"},"@text":"biosynthesis"}]},{"DescriptorName":{"@attributes":{"UI":"D011994","MajorTopicYN":"N"},"@text":"Recombinant Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D015398","MajorTopicYN":"N"},"@text":"Signal Transduction"}},{"DescriptorName":{"@attributes":{"UI":"D014157","MajorTopicYN":"N"},"@text":"Transcription Factors"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D014158","MajorTopicYN":"Y"},"@text":"Transcription, Genetic"}},{"DescriptorName":{"@attributes":{"UI":"D016212","MajorTopicYN":"N"},"@text":"Transforming Growth Factor beta"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D014982","MajorTopicYN":"N"},"@text":"Xenopus laevis"}}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"2000","Month":"3","Day":"11"},{"@attributes":{"PubStatus":"medline"},"Year":"2000","Month":"3","Day":"11","Hour":"0","Minute":"1"},{"@attributes":{"PubStatus":"entrez"},"Year":"2000","Month":"3","Day":"11","Hour":"0","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10678167"},{"@attributes":{"IdType":"doi"},"@text":"10.1016\/s1097-2765(00)80401-3"},{"@attributes":{"IdType":"pii"},"@text":"S1097-2765(00)80401-3"}]}}}}