{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"10690529"},"DateCompleted":{"Year":"2000","Month":"03","Day":"28"},"DateRevised":{"Year":"2008","Month":"11","Day":"21"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"1078-0432"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"6","Issue":"2","PubDate":{"Year":"2000","Month":"Feb"}},"Title":"Clinical cancer research : an official journal of the American Association for Cancer Research","ISOAbbreviation":"Clin Cancer Res"},"ArticleTitle":"Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene.","Pagination":{"StartPage":"493","EndPage":"497","MedlinePgn":"493-7"},"Abstract":{"AbstractText":["Previous studies demonstrated that the GBX2 homeobox gene is consistently overexpressed in cultured human prostate cancer cell lines. In this study, the human GBX2 cDNA was cloned and a quantitative reverse transcription-PCR method used to demonstrate that GBX2 mRNA expression is enhanced in approximately 70% of human prostate cancer tissues compared with normal human prostate tissues. Purified recombinant GBX2 protein binds specifically to an ATTA motif within the promoter of the interleukin 6 (IL-6) gene. Using an antisense approach, down-regulation of the expression of GBX2 correlated with decreased expression of IL-6 and an inhibition of tumorigenicity of PC3 human prostate cancer cells. In addition, in vitro growth of the antisense clones was partially restored by exogenous addition of recombinant IL-6 protein to the culture media. These data demonstrated that enhanced GBX2 expression results in a stimulation of malignant growth of prostate cancer cells and that part of this stimulation involves up-regulation in the transcription of the IL-6 gene."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Gao","ForeName":"A C","Initials":"AC","AffiliationInfo":[{"Affiliation":"University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA. gaoac@msx.upmc.edu"}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Lou","ForeName":"W","Initials":"W"},{"@attributes":{"ValidYN":"Y"},"LastName":"Isaacs","ForeName":"J T","Initials":"JT"}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"CA 58236","Acronym":"CA","Agency":"NCI NIH HHS","Country":"United States"},{"GrantID":"P30CA47904","Acronym":"CA","Agency":"NCI NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Clin Cancer Res","NlmUniqueID":"9502500","ISSNLinking":"1078-0432"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C084958"},"@text":"GBX2 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C513182"},"@text":"Gbx2 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D015850"},"@text":"Interleukin-6"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D012333"},"@text":"RNA, Messenger"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D011994"},"@text":"Recombinant Proteins"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D002455","MajorTopicYN":"N"},"@text":"Cell Division"}},{"DescriptorName":{"@attributes":{"UI":"D003001","MajorTopicYN":"N"},"@text":"Cloning, Molecular"}},{"DescriptorName":{"@attributes":{"UI":"D015972","MajorTopicYN":"Y"},"@text":"Gene Expression Regulation, Neoplastic"}},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"N"},"@text":"Homeodomain Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D015850","MajorTopicYN":"N"},"@text":"Interleukin-6"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D008297","MajorTopicYN":"N"},"@text":"Male"}},{"DescriptorName":{"@attributes":{"UI":"D051379","MajorTopicYN":"N"},"@text":"Mice"}},{"DescriptorName":{"@attributes":{"UI":"D011401","MajorTopicYN":"N"},"@text":"Promoter Regions, Genetic"}},{"DescriptorName":{"@attributes":{"UI":"D011471","MajorTopicYN":"N"},"@text":"Prostatic Neoplasms"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000473","MajorTopicYN":"Y"},"@text":"pathology"},{"@attributes":{"UI":"Q000601","MajorTopicYN":"N"},"@text":"surgery"}]},{"DescriptorName":{"@attributes":{"UI":"D012333","MajorTopicYN":"N"},"@text":"RNA, Messenger"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D011994","MajorTopicYN":"N"},"@text":"Recombinant Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D016415","MajorTopicYN":"N"},"@text":"Sequence Alignment"}},{"DescriptorName":{"@attributes":{"UI":"D017386","MajorTopicYN":"N"},"@text":"Sequence Homology, Amino Acid"}},{"DescriptorName":{"@attributes":{"UI":"D014158","MajorTopicYN":"Y"},"@text":"Transcription, Genetic"}},{"DescriptorName":{"@attributes":{"UI":"D014162","MajorTopicYN":"N"},"@text":"Transfection"}},{"DescriptorName":{"@attributes":{"UI":"D014407","MajorTopicYN":"N"},"@text":"Tumor Cells, Cultured"}}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"2000","Month":"2","Day":"26","Hour":"9","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2000","Month":"4","Day":"1","Hour":"9","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2000","Month":"2","Day":"26","Hour":"9","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10690529"}]}}}}