{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"12196542"},"DateCompleted":{"Year":"2002","Month":"12","Day":"09"},"DateRevised":{"Year":"2021","Month":"02","Day":"06"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0021-9258"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"277","Issue":"43","PubDate":{"Year":"2002","Month":"Oct","Day":"25"}},"Title":"The Journal of biological chemistry","ISOAbbreviation":"J Biol Chem"},"ArticleTitle":"PDZ7 of glutamate receptor interacting protein binds to its target via a novel hydrophobic surface area.","Pagination":{"StartPage":"41140","EndPage":"41146","MedlinePgn":"41140-6"},"Abstract":{"AbstractText":["Glutamate receptor interacting protein 1 (GRIP1) is a scaffold protein composed of seven PDZ (Postsynaptic synaptic density-95\/Discs large\/Zona occludens-1) domains. The protein plays important roles in the synaptic targeting of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. The interaction between GRIP1 PDZ7 and a Ras guanine nucleotide exchange factor, GRASP-1, regulates synaptic distribution of AMPA receptors. Here, we describe the three-dimensional structure of GRIP1 PDZ7 determined by NMR spectroscopy. GRIP1 PDZ7 contains a closed carboxyl group-binding pocket and a narrow alphaB\/betaB-groove that is not likely to bind to classical PDZ ligands. Unexpectedly, GRIP1 PDZ7 contains a large solvent-exposed hydrophobic surface at a site distinct from the conventional ligand-binding alphaB\/betaB-groove. NMR titration experiments show that GRIP1 PDZ7 binds to GRASP-1 via this hydrophobic surface. Our data uncover a novel PDZ domain-mediated protein interaction mode that may be responsible for multimerization of other PDZ domain-containing scaffold proteins."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Feng","ForeName":"Wei","Initials":"W","AffiliationInfo":[{"Affiliation":"Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Fan","ForeName":"Jing-Song","Initials":"JS"},{"@attributes":{"ValidYN":"Y"},"LastName":"Jiang","ForeName":"Ming","Initials":"M"},{"@attributes":{"ValidYN":"Y"},"LastName":"Shi","ForeName":"Ya-Wei","Initials":"YW"},{"@attributes":{"ValidYN":"Y"},"LastName":"Zhang","ForeName":"Mingjie","Initials":"M"}]},"Language":["eng"],"DataBankList":{"@attributes":{"CompleteYN":"Y"},"DataBank":[{"DataBankName":"PDB","AccessionNumberList":{"AccessionNumber":["1M5Z"]}}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2002","Month":"08","Day":"23"}]},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"J Biol Chem","NlmUniqueID":"2985121R","ISSNLinking":"0021-9258"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D002352"},"@text":"Carrier Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491308"},"@text":"Grip1 protein, rat"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D047908"},"@text":"Intracellular Signaling Peptides and Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D009419"},"@text":"Nerve Tissue Proteins"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000595","MajorTopicYN":"N"},"@text":"Amino Acid Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D001665","MajorTopicYN":"N"},"@text":"Binding Sites"}},{"DescriptorName":{"@attributes":{"UI":"D002352","MajorTopicYN":"N"},"@text":"Carrier Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D047908","MajorTopicYN":"N"},"@text":"Intracellular Signaling Peptides and Proteins"}},{"DescriptorName":{"@attributes":{"UI":"D008958","MajorTopicYN":"N"},"@text":"Models, Molecular"}},{"DescriptorName":{"@attributes":{"UI":"D008969","MajorTopicYN":"N"},"@text":"Molecular Sequence Data"}},{"DescriptorName":{"@attributes":{"UI":"D009419","MajorTopicYN":"N"},"@text":"Nerve Tissue Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D019906","MajorTopicYN":"N"},"@text":"Nuclear Magnetic Resonance, Biomolecular"}},{"DescriptorName":{"@attributes":{"UI":"D011485","MajorTopicYN":"N"},"@text":"Protein Binding"}},{"DescriptorName":{"@attributes":{"UI":"D011487","MajorTopicYN":"N"},"@text":"Protein Conformation"}},{"DescriptorName":{"@attributes":{"UI":"D051381","MajorTopicYN":"N"},"@text":"Rats"}},{"DescriptorName":{"@attributes":{"UI":"D017386","MajorTopicYN":"N"},"@text":"Sequence Homology, Amino Acid"}}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"2002","Month":"8","Day":"28","Hour":"10","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2002","Month":"12","Day":"10","Hour":"4","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2002","Month":"8","Day":"28","Hour":"10","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"12196542"},{"@attributes":{"IdType":"doi"},"@text":"10.1074\/jbc.M207206200"},{"@attributes":{"IdType":"pii"},"@text":"S0021-9258(19)72230-3"}]}}}}