{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"12367504"},"DateCompleted":{"Year":"2002","Month":"11","Day":"04"},"DateRevised":{"Year":"2025","Month":"05","Day":"29"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0896-6273"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"36","Issue":"1","PubDate":{"Year":"2002","Month":"Sep","Day":"26"}},"Title":"Neuron","ISOAbbreviation":"Neuron"},"ArticleTitle":"Changing requirements for Gbx2 in development of the cerebellum and maintenance of the mid\/hindbrain organizer.","Pagination":{"StartPage":"31","EndPage":"43","MedlinePgn":"31-43"},"Abstract":{"AbstractText":["We examined whether Gbx2 is required after embryonic day 9 (E9) to repress Otx2 in the cerebellar anlage and position the midbrain\/hindbrain organizer. In contrast to Gbx2 null mutants, mice lacking Gbx2 in rhombomere 1 (r1) after E9 (Gbx2-CKO) are viable and develop a cerebellum. A Gbx2-independent pathway can repress Otx2 in r1 after E9. Mid\/hindbrain organizer gene expression, however, continues to be dependent on Gbx2. We found that Fgf8 expression normally correlates with the isthmus where cells undergo low proliferation and that in Gbx2-CKO mutants this domain is expanded. We propose that Fgf8 permits lateral cerebellar development through repression of Otx2 and also suppresses medial cerebellar growth in Gbx2-CKO embryos. Our work has uncovered distinct requirements for Gbx2 during cerebellum formation and provided a model for how a transcription factor can play multiple roles during development."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Li","ForeName":"James Y H","Initials":"JY","AffiliationInfo":[{"Affiliation":"Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Lao","ForeName":"Zhimin","Initials":"Z"},{"@attributes":{"ValidYN":"Y"},"LastName":"Joyner","ForeName":"Alexandra L","Initials":"AL"}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"F32 NS041702","Acronym":"NS","Agency":"NINDS NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Neuron","NlmUniqueID":"8809320","ISSNLinking":"0896-6273"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C513182"},"@text":"Gbx2 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C080331"},"@text":"Hoxa3 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D009419"},"@text":"Nerve Tissue Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D051857"},"@text":"Otx Transcription Factors"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C497660"},"@text":"Otx2 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D011518"},"@text":"Proto-Oncogene Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D015534"},"@text":"Trans-Activators"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D051153"},"@text":"Wnt Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D029961"},"@text":"Zebrafish Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C513181"},"@text":"gbx2 protein, zebrafish"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C489831"},"@text":"hoxa2b protein, zebrafish"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D019521","MajorTopicYN":"N"},"@text":"Body Patterning"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D002454","MajorTopicYN":"N"},"@text":"Cell Differentiation"},"QualifierName":[{"@attributes":{"UI":"Q000502","MajorTopicYN":"N"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D002531","MajorTopicYN":"N"},"@text":"Cerebellum"},"QualifierName":[{"@attributes":{"UI":"Q000002","MajorTopicYN":"Y"},"@text":"abnormalities"},{"@attributes":{"UI":"Q000166","MajorTopicYN":"N"},"@text":"cytology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D005260","MajorTopicYN":"N"},"@text":"Female"}},{"DescriptorName":{"@attributes":{"UI":"D005333","MajorTopicYN":"N"},"@text":"Fetus"}},{"DescriptorName":{"@attributes":{"UI":"D018507","MajorTopicYN":"N"},"@text":"Gene Expression Regulation, Developmental"},"QualifierName":[{"@attributes":{"UI":"Q000502","MajorTopicYN":"N"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"N"},"@text":"Homeodomain 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