{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"15059951"},"DateCompleted":{"Year":"2004","Month":"07","Day":"14"},"DateRevised":{"Year":"2025","Month":"01","Day":"03"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0013-7227"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"145","Issue":"7","PubDate":{"Year":"2004","Month":"Jul"}},"Title":"Endocrinology","ISOAbbreviation":"Endocrinology"},"ArticleTitle":"Concerted regulation of the porcine steroidogenic acute regulatory protein gene promoter activity by follicle-stimulating hormone and insulin-like growth factor I in granulosa cells involves GATA-4 and CCAAT\/enhancer binding protein beta.","Pagination":{"StartPage":"3122","EndPage":"3134","MedlinePgn":"3122-34"},"Abstract":{"AbstractText":["We previously demonstrated that FSH alone or in combination with IGF-I activated the porcine steroidogenic acute regulatory protein gene promoter in a concerted manner in primary cultures of granulosa cells. Studies were undertaken to further delineate cis- and trans-acting elements of the porcine promoter and mechanisms mediating FSH stimulation and its augmentation by IGF-I. Mutation of several putative regulatory elements localized hormone-stimulated activity to the highly conserved GATA site and identified novel nucleotides downstream as a functional CCAAT\/enhancer binding protein (C\/EBP)beta site. In granulosa cell nuclear extracts, GATA-4 and C\/EBPbeta formed a high-molecular-weight complex with an oligonucleotide spanning -76 to -32 bp of the porcine promoter. The intensity of this high-molecular-weight complex was increased in granulosa cell nuclear extracts by treatment with FSH alone and was enhanced with the combination of FSH and IGF-I at 2-3 h of treatment. GATA-4 and C\/EBPbeta proteins were uniformly expressed with all treatments at time points associated with increased DNA binding. Treatment (2 h) with FSH alone or FSH + IGF-I increased phosphorylation of GATA-4 on a protein kinase A consensus site. The 38-kDa isoform of C\/EBPbeta exhibited greater phosphorylation with FSH + IGF-I treatment. Porcine luteal cell nuclear extracts also demonstrated GATA-4 and C\/EBPbeta binding to the -76 to -32 bp region of the promoter providing evidence for their cooperation in vivo. These data indicate that GATA-4 and C\/EBPbeta are both required for FSH +\/- IGF-I stimulation of the porcine steroidogenic acute regulatory protein gene promoter in homologous granulosa cell cultures."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"LaVoie","ForeName":"Holly A","Initials":"HA","AffiliationInfo":[{"Affiliation":"Department of Cell and Developmental Biology and Anatomy, Building 1, University of South Carolina School of Medicine, Columbia, South Carolina 29208, USA. hlavoie@med.sc.edu."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Singh","ForeName":"Dheer","Initials":"D"},{"@attributes":{"ValidYN":"Y"},"LastName":"Hui","ForeName":"Yvonne Y","Initials":"YY"}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"HD-038945","Acronym":"HD","Agency":"NICHD NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2004","Month":"04","Day":"01"}]},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Endocrinology","NlmUniqueID":"0375040","ISSNLinking":"0013-7227"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D022782"},"@text":"CCAAT-Enhancer-Binding Protein-beta"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D004268"},"@text":"DNA-Binding Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D050991"},"@text":"GATA4 Transcription Factor"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D010750"},"@text":"Phosphoproteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D014157"},"@text":"Transcription Factors"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D000098682"},"@text":"Steroidogenic Acute Regulatory Protein"}},{"RegistryNumber":"67763-96-6","NameOfSubstance":{"@attributes":{"UI":"D007334"},"@text":"Insulin-Like Growth Factor I"}},{"RegistryNumber":"9002-68-0","NameOfSubstance":{"@attributes":{"UI":"D005640"},"@text":"Follicle Stimulating Hormone"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D001483","MajorTopicYN":"N"},"@text":"Base Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D022782","MajorTopicYN":"N"},"@text":"CCAAT-Enhancer-Binding Protein-beta"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002478","MajorTopicYN":"N"},"@text":"Cells, Cultured"}},{"DescriptorName":{"@attributes":{"UI":"D004268","MajorTopicYN":"N"},"@text":"DNA-Binding Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D005260","MajorTopicYN":"N"},"@text":"Female"}},{"DescriptorName":{"@attributes":{"UI":"D005640","MajorTopicYN":"N"},"@text":"Follicle Stimulating Hormone"},"QualifierName":[{"@attributes":{"UI":"Q000494","MajorTopicYN":"Y"},"@text":"pharmacology"}]},{"DescriptorName":{"@attributes":{"UI":"D050991","MajorTopicYN":"N"},"@text":"GATA4 Transcription Factor"}},{"DescriptorName":{"@attributes":{"UI":"D006107","MajorTopicYN":"N"},"@text":"Granulosa Cells"},"QualifierName":[{"@attributes":{"UI":"Q000166","MajorTopicYN":"N"},"@text":"cytology"},{"@attributes":{"UI":"Q000187","MajorTopicYN":"N"},"@text":"drug effects"},{"@attributes":{"UI":"Q000502","MajorTopicYN":"Y"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D007334","MajorTopicYN":"N"},"@text":"Insulin-Like Growth Factor I"},"QualifierName":[{"@attributes":{"UI":"Q000494","MajorTopicYN":"Y"},"@text":"pharmacology"}]},{"DescriptorName":{"@attributes":{"UI":"D008969","MajorTopicYN":"N"},"@text":"Molecular Sequence Data"}},{"DescriptorName":{"@attributes":{"UI":"D016297","MajorTopicYN":"N"},"@text":"Mutagenesis, Site-Directed"}},{"DescriptorName":{"@attributes":{"UI":"D010750","MajorTopicYN":"N"},"@text":"Phosphoproteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D010766","MajorTopicYN":"N"},"@text":"Phosphorylation"}},{"DescriptorName":{"@attributes":{"UI":"D011401","MajorTopicYN":"N"},"@text":"Promoter Regions, Genetic"},"QualifierName":[{"@attributes":{"UI":"Q000502","MajorTopicYN":"N"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D034421","MajorTopicYN":"N"},"@text":"Sus scrofa"}},{"DescriptorName":{"@attributes":{"UI":"D014157","MajorTopicYN":"N"},"@text":"Transcription Factors"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D014162","MajorTopicYN":"N"},"@text":"Transfection"}},{"DescriptorName":{"@attributes":{"UI":"D000098682","MajorTopicYN":"N"},"@text":"Steroidogenic Acute Regulatory Protein"}}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"2004","Month":"4","Day":"3","Hour":"5","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2004","Month":"7","Day":"15","Hour":"5","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2004","Month":"4","Day":"3","Hour":"5","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15059951"},{"@attributes":{"IdType":"doi"},"@text":"10.1210\/en.2003-1719"},{"@attributes":{"IdType":"pii"},"@text":"en.2003-1719"}]}}}}