{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"15226318"},"DateCompleted":{"Year":"2004","Month":"10","Day":"19"},"DateRevised":{"Year":"2021","Month":"02","Day":"06"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0021-9258"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"279","Issue":"37","PubDate":{"Year":"2004","Month":"Sep","Day":"10"}},"Title":"The Journal of biological chemistry","ISOAbbreviation":"J Biol Chem"},"ArticleTitle":"A four PDZ domain-containing splice variant form of GRIP1 is localized in GABAergic and glutamatergic synapses in the brain.","Pagination":{"StartPage":"38978","EndPage":"38990","MedlinePgn":"38978-90"},"Abstract":{"AbstractText":["We have isolated, from a rat brain cDNA library, a clone corresponding to a 2779-bp cDNA encoding a novel splice form of the glutamate receptor interacting protein-1 (GRIP1). We call this 696-amino acid splice form GRIP1c 4-7 to differentiate it from longer splice forms of GRIP1a\/b containing seven PDZ domains. The four PDZ domains of GRIP1c 4-7 are identical to PDZ domains 4-7 of GRIP1a\/b. GRIP1c 4-7 also contains 35 amino acids at the N terminus and 12 amino acids at the C terminus that are different from GRIP1a\/b. In transfected HEK293 cells, a majority of GRIP1c 4-7 was associated with the plasma membrane. GRIP1c 4-7 interacted with GluR2\/3 subunits of the alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptor. In low density hippocampal cultures, GRIP1c 4-7 clusters colocalized with GABAergic (where GABA is gamma-aminobutyric acid) and glutamatergic synapses, although a higher percentage of GRIP1c 4-7 clusters colocalized with gamma-aminobutyric acid, type A, receptor (GABA(A)R) clusters than with alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptor clusters. Transfection of hippocampal neurons with hemagglutinin-tagged GRIP1c 4-7 showed that it could target to the postsynaptic complex of GABAergic synapses colocalizing with GABA(A)R clusters. GRIP1c 4-7-specific antibodies, which did not recognize previously described splice forms of GRIP1, recognized a 75-kDa protein that is enriched in a postsynaptic density fraction isolated from rat brain. EM immunocytochemistry experiments showed that in intact brain GRIP1c 4-7 concentrates at postsynaptic complexes of both type I glutamatergic and type II GABAergic synapses although it is also presynaptically localized. These results indicate that GRIP1c 4-7 plays a role not only in glutamatergic synapses but also in GABAergic synapses."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Charych","ForeName":"Erik I","Initials":"EI","AffiliationInfo":[{"Affiliation":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Yu","ForeName":"Wendou","Initials":"W"},{"@attributes":{"ValidYN":"Y"},"LastName":"Li","ForeName":"Rongwen","Initials":"R"},{"@attributes":{"ValidYN":"Y"},"LastName":"Serwanski","ForeName":"David R","Initials":"DR"},{"@attributes":{"ValidYN":"Y"},"LastName":"Miralles","ForeName":"Celia P","Initials":"CP"},{"@attributes":{"ValidYN":"Y"},"LastName":"Li","ForeName":"Xuejing","Initials":"X"},{"@attributes":{"ValidYN":"Y"},"LastName":"Yang","ForeName":"Bih Y","Initials":"BY"},{"@attributes":{"ValidYN":"Y"},"LastName":"Pinal","ForeName":"Noelia","Initials":"N"},{"@attributes":{"ValidYN":"Y"},"LastName":"Walikonis","ForeName":"Randall","Initials":"R"},{"@attributes":{"ValidYN":"Y"},"LastName":"De Blas","ForeName":"Angel L","Initials":"AL"}]},"Language":["eng"],"DataBankList":{"@attributes":{"CompleteYN":"Y"},"DataBank":[{"DataBankName":"GENBANK","AccessionNumberList":{"AccessionNumber":["AY437398"]}}]},"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"NS38752","Acronym":"NS","Agency":"NINDS NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2004","Month":"06","Day":"29"}]},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"J Biol Chem","NlmUniqueID":"2985121R","ISSNLinking":"0021-9258"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D020121"},"@text":"5' Untranslated Regions"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D002352"},"@text":"Carrier Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018076"},"@text":"DNA, Complementary"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018683"},"@text":"Excitatory Amino Acid Agents"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018682"},"@text":"GABA Agents"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491310"},"@text":"GRIP1 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491308"},"@text":"Grip1 protein, rat"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D047908"},"@text":"Intracellular Signaling Peptides and Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D009419"},"@text":"Nerve Tissue Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018091"},"@text":"Receptors, AMPA"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D020121","MajorTopicYN":"N"},"@text":"5' Untranslated Regions"}},{"DescriptorName":{"@attributes":{"UI":"D017398","MajorTopicYN":"N"},"@text":"Alternative Splicing"}},{"DescriptorName":{"@attributes":{"UI":"D000595","MajorTopicYN":"N"},"@text":"Amino Acid Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D001921","MajorTopicYN":"N"},"@text":"Brain"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002352","MajorTopicYN":"N"},"@text":"Carrier 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