{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"16539648"},"DateCompleted":{"Year":"2006","Month":"06","Day":"22"},"DateRevised":{"Year":"2017","Month":"11","Day":"16"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0022-3042"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"97","Issue":"3","PubDate":{"Year":"2006","Month":"May"}},"Title":"Journal of neurochemistry","ISOAbbreviation":"J Neurochem"},"ArticleTitle":"Identification and characterization of two novel splice forms of GRIP1 in the rat brain.","Pagination":{"StartPage":"884","EndPage":"898","MedlinePgn":"884-98"},"Abstract":{"AbstractText":["We cloned two novel alternatively-spliced mRNA isoforms of glutamate receptor interacting protein 1 (GRIP1) which we named GRIP1d and GRIP1e 4-7. GRIP1d is a 135 kDa, 7-PDZ-domain variant of GRIP1, containing the 12 amino acid C-terminus originally described for the 4-PDZ-domain GRIP1c 4-7. GRIP1e 4-7 is a 75 kDa 4-PDZ-domain variant of GRIP1, containing the 12 amino acid C-terminus originally described for the 7-PDZ-domain GRIP1a\/b. Northern blots indicated that GRIP1d mRNA is 5.1 kb long and abundant in brain. An antibody to the C-terminus of the 75 kDa GRIP1c 4-7 also recognized an abundant 135 kDa protein, consistent with the predicted size of GRIP1d. Similarly, an antibody to the C-terminus of the 135 kDa GRIP1a\/b also recognized a low abundance 75 kDa protein, consistent with the predicted size of GRIP1e 4-7. Immunocytochemistry of hippocampal cultures and intact brain using these antibodies showed that (i) these isoforms are present in both GABAergic and glutamatergic synapses, and (ii) the isoforms co-localize in individual synapses. While GRIP1a\/b isoforms are abundant in interneurons and highly concentrated in GABAergic presynaptic terminals, the isoforms recognized by the antibody to the C-terminus common to GRIP1c 4-7 and GRIP1d are much less abundant in interneurons and preferentially concentrate at the postsynaptic complex."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Charych","ForeName":"Erik I","Initials":"EI","AffiliationInfo":[{"Affiliation":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269-3156, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Li","ForeName":"Rongwen","Initials":"R"},{"@attributes":{"ValidYN":"Y"},"LastName":"Serwanski","ForeName":"David R","Initials":"DR"},{"@attributes":{"ValidYN":"Y"},"LastName":"Li","ForeName":"Xuejing","Initials":"X"},{"@attributes":{"ValidYN":"Y"},"LastName":"Miralles","ForeName":"Celia P","Initials":"CP"},{"@attributes":{"ValidYN":"Y"},"LastName":"Pinal","ForeName":"Noelia","Initials":"N"},{"@attributes":{"ValidYN":"Y"},"LastName":"De Blas","ForeName":"Angel L","Initials":"AL"}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"NS38752","Acronym":"NS","Agency":"NINDS NIH HHS","Country":"United States"},{"GrantID":"NS39287","Acronym":"NS","Agency":"NINDS NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D003160"},"@text":"Comparative Study"},{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D052061"},"@text":"Research Support, N.I.H., Extramural"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2006","Month":"03","Day":"15"}]},"MedlineJournalInfo":{"Country":"England","MedlineTA":"J Neurochem","NlmUniqueID":"2985190R","ISSNLinking":"0022-3042"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D002352"},"@text":"Carrier Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D000076122"},"@text":"Disks Large Homolog 4 Protein"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C501449"},"@text":"Dlg4 protein, rat"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C491308"},"@text":"Grip1 protein, rat"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D047908"},"@text":"Intracellular Signaling Peptides and Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D008565"},"@text":"Membrane Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D009419"},"@text":"Nerve Tissue Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D020033"},"@text":"Protein Isoforms"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D050598"},"@text":"Vesicular Glutamate Transport Protein 1"}},{"RegistryNumber":"EC 4.1.1.15","NameOfSubstance":{"@attributes":{"UI":"D005968"},"@text":"Glutamate Decarboxylase"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D017398","MajorTopicYN":"N"},"@text":"Alternative Splicing"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D000595","MajorTopicYN":"N"},"@text":"Amino Acid Sequence"}},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D015152","MajorTopicYN":"N"},"@text":"Blotting, Northern"},"QualifierName":[{"@attributes":{"UI":"Q000379","MajorTopicYN":"N"},"@text":"methods"}]},{"DescriptorName":{"@attributes":{"UI":"D001921","MajorTopicYN":"N"},"@text":"Brain"},"QualifierName":[{"@attributes":{"UI":"Q000166","MajorTopicYN":"N"},"@text":"cytology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"},{"@attributes":{"UI":"Q000648","MajorTopicYN":"N"},"@text":"ultrastructure"}]},{"DescriptorName":{"@attributes":{"UI":"D002352","MajorTopicYN":"N"},"@text":"Carrier Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002478","MajorTopicYN":"N"},"@text":"Cells, Cultured"}},{"DescriptorName":{"@attributes":{"UI":"D003001","MajorTopicYN":"N"},"@text":"Cloning, Molecular"},"QualifierName":[{"@attributes":{"UI":"Q000379","MajorTopicYN":"N"},"@text":"methods"}]},{"DescriptorName":{"@attributes":{"UI":"D000076122","MajorTopicYN":"N"},"@text":"Disks Large Homolog 4 Protein"}},{"DescriptorName":{"@attributes":{"UI":"D004622","MajorTopicYN":"N"},"@text":"Embryo, Mammalian"}},{"DescriptorName":{"@attributes":{"UI":"D005968","MajorTopicYN":"N"},"@text":"Glutamate Decarboxylase"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D006624","MajorTopicYN":"N"},"@text":"Hippocampus"},"QualifierName":[{"@attributes":{"UI":"Q000166","MajorTopicYN":"N"},"@text":"cytology"}]},{"DescriptorName":{"@attributes":{"UI":"D007150","MajorTopicYN":"N"},"@text":"Immunohistochemistry"},"QualifierName":[{"@attributes":{"UI":"Q000379","MajorTopicYN":"N"},"@text":"methods"}]},{"DescriptorName":{"@attributes":{"UI":"D047908","MajorTopicYN":"N"},"@text":"Intracellular Signaling Peptides and 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Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D009474","MajorTopicYN":"N"},"@text":"Neurons"},"QualifierName":[{"@attributes":{"UI":"Q000166","MajorTopicYN":"N"},"@text":"cytology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D020411","MajorTopicYN":"N"},"@text":"Oligonucleotide Array Sequence Analysis"},"QualifierName":[{"@attributes":{"UI":"Q000379","MajorTopicYN":"N"},"@text":"methods"}]},{"DescriptorName":{"@attributes":{"UI":"D020033","MajorTopicYN":"N"},"@text":"Protein 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1"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"2006","Month":"3","Day":"17","Hour":"9","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2006","Month":"6","Day":"23","Hour":"9","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2006","Month":"3","Day":"17","Hour":"9","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16539648"},{"@attributes":{"IdType":"doi"},"@text":"10.1111\/j.1471-4159.2006.03795.x"},{"@attributes":{"IdType":"pii"},"@text":"JNC3795"}]}}}}