{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"PubMed-not-MEDLINE","Owner":"NLM"},"PMID":{"@attributes":{"Version":"1"},"@text":"19524703"},"DateCompleted":{"Year":"2009","Month":"12","Day":"15"},"DateRevised":{"Year":"2025","Month":"05","Day":"29"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"1567-133X"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"9","Issue":"6","PubDate":{"Year":"2009","Month":"Sep"}},"Title":"Gene expression patterns : GEP","ISOAbbreviation":"Gene Expr Patterns"},"ArticleTitle":"fezf2 expression delineates cells with proliferative potential and expressing markers of neural stem cells in the adult zebrafish brain.","Pagination":{"StartPage":"411","EndPage":"422","MedlinePgn":"411-22"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1016\/j.gep.2009.06.002"}],"Abstract":{"AbstractText":["Fezf2 (also known as Fezl, ZNF312, or Zfp312) is an evolutionarily conserved forebrain-specific zinc finger transcription factor that is expressed during development and is implicated in patterning as well as neurogenesis in both zebrafish and mice. Despite these findings, the expression of fezf2 in the adult brain has not been well characterized, and fezf2 function in the adult brain remains unknown. The zebrafish has recently emerged as a new model system to study adult neurogenesis, given its similarity to mammalian systems and enhanced capability of undergoing adult neurogenesis. Through RNA in situ hybridization and using a fezf2 promoter-driven GFP transgenic line, we present data showing that fezf2 is expressed in radial glial progenitor cells of the telencephalic ventricular zone in the adult zebrafish brain, which co-express markers of neural stem cells and proliferation. Additionally, we identify the preoptic region and the hypothalamus as fezf2-expressing neurogenic regions in the adult zebrafish brain, where fezf2 labels progenitor cells as well as postmitotic neurons. Our findings establish Fezf2 as a novel marker for adult telencephalic ventricular progenitor cells that express markers of neural stem cells in zebrafish and lay a critical foundation for future investigation of Fezf2 function in the maintenance and differentiation of neural stem cells in the adult vertebrate brain."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Berberoglu","ForeName":"Michael A","Initials":"MA","AffiliationInfo":[{"Affiliation":"Programs in Developmental Biology, Neuroscience and Human Genetics, Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158-2811, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Dong","ForeName":"Zhiqiang","Initials":"Z"},{"@attributes":{"ValidYN":"Y"},"LastName":"Mueller","ForeName":"Thomas","Initials":"T"},{"@attributes":{"ValidYN":"Y"},"LastName":"Guo","ForeName":"Su","Initials":"S"}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"R01 NS042626","Acronym":"NS","Agency":"NINDS NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2009","Month":"06","Day":"12"}]},"MedlineJournalInfo":{"Country":"Netherlands","MedlineTA":"Gene Expr Patterns","NlmUniqueID":"101167473","ISSNLinking":"1567-133X"}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2009","Month":"4","Day":"10"},{"@attributes":{"PubStatus":"revised"},"Year":"2009","Month":"6","Day":"3"},{"@attributes":{"PubStatus":"accepted"},"Year":"2009","Month":"6","Day":"8"},{"@attributes":{"PubStatus":"entrez"},"Year":"2009","Month":"6","Day":"16","Hour":"9","Minute":"0"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2009","Month":"6","Day":"16","Hour":"9","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2009","Month":"6","Day":"16","Hour":"9","Minute":"1"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"2010","Month":"9","Day":"1"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"19524703"},{"@attributes":{"IdType":"mid"},"@text":"NIHMS124330"},{"@attributes":{"IdType":"pmc"},"@text":"PMC2746713"},{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.gep.2009.06.002"},{"@attributes":{"IdType":"pii"},"@text":"S1567-133X(09)00066-0"}]},"ReferenceList":[{"Reference":[{"Citation":"Adolf B, Chapouton P, Lam CS, Topp S, Tannh\u00e4user B, Str\u00e4hle U, G\u00f6tz M, Bally-Cuif L. Conserved and acquired features of adult neurogenesis in the zebrafish telencephalon. Dev. Biol. 2006;295:278\u2013293.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16828638"}]}},{"Citation":"Alvarez-Buylla A, Garcia-Verdugo M. Neurogenesis in adult subventricular zone. J. Neurosci. 2002;22:629\u2013634.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pmc"},"@text":"PMC6758521"},{"@attributes":{"IdType":"pubmed"},"@text":"11826091"}]}},{"Citation":"Byrd CA, Brunjes PC. Addition of new cells to the olfactory bulb of adult zebrafish. Ann. N. Y. Acad. Sci. 1998;855:274\u2013276.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"9929621"}]}},{"Citation":"Chapouton P, Adolf B, Leucht C, Tannh\u00e4user B, Ryu S, Driever W, Bally-Cuif L. her5 expression reveals a pool of neural stem cells in the adult zebrafish midbrain. Development. 2006;133:4293\u20134303.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"17038515"}]}},{"Citation":"Chapouton P, Jagasia R, Bally-Cuif L. Adult neurogenesis in non-mammalian vertebrates. BioEssays. 2007;29:745\u2013757.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"17621643"}]}},{"Citation":"Chen B, Schaevitz LR, McConnell SK. Fezl regulates the differentiation and axon targeting of layer 5 subcortical projection neurons in cerebral cortex. Proc. Natl. Acad. Sci. USA. 2005a;102:17184\u201317189.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pmc"},"@text":"PMC1282569"},{"@attributes":{"IdType":"pubmed"},"@text":"16284245"}]}},{"Citation":"Chen JG, Rasin MR, Kwan KY, Sestan N. zfp312 is required for subcortical axonal projections and dendritic morphology of deep-layer pyramidal neurons of the cerebral cortex. Proc. Natl. Acad. Sci. USA. 2005b;102:17792\u201317797.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pmc"},"@text":"PMC1308928"},{"@attributes":{"IdType":"pubmed"},"@text":"16314561"}]}},{"Citation":"Coskun V, Wu H, Blanchi B, Tsao S, Kim K, Zhao J, Biancotti JC, Hutnick L, Krueger RC, Jr, Fan G, de Vellis J, Sun YE. CD133+ neural stem cells in the ependyma of mammalian postnatal forebrain. Proc. Natl. Acad. Sci. USA. 2008;105:1026\u20131031.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pmc"},"@text":"PMC2242680"},{"@attributes":{"IdType":"pubmed"},"@text":"18195354"}]}},{"Citation":"Doetsch F, Caille I, Lim DA, Garcia-Verdugo JM, Alvarez-Buylla A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell. 1999;97:703\u2013716.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10380923"}]}},{"Citation":"Eisen JS. Zebrafish make a big splash. Cell. 1996;87:969\u2013977.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"8978602"}]}},{"Citation":"Episkopou V. Sox2 functions in adult neural stem cells. Trends Neurosci. 2005;28:219\u2013221.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15866195"}]}},{"Citation":"Ferri AL, Cavallaro M, Braida D, Di Cristofano A, Canta A, Vezzani A, Ottolenghi S, Pandolfi PP, Sala M, DeBiasi S, Nicolis SK. Sox2 deficiency causes neurodegeneration and impaired neurogenesis in the adult mouse brain. Development. 2004;131:3805\u20133819.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15240551"}]}},{"Citation":"Gage FH. Mammalian neural stem cells. Science. 2000;287:1433\u20131438.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10688783"}]}},{"Citation":"Garcia A, Doan N, Imura T, Bush T, Sofroniew M. GFAP expressing progenitors are the principal source of constitutive neurogenesis in adult mouse forebrain. Nat. Neurosci. 2004;7:1233\u20131241.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15494728"}]}},{"Citation":"Goldman S. Glia as neural progenitor cells. Trends Neurosci. 2003;26:590\u2013596.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"14585598"}]}},{"Citation":"Grandel H, Kaslin J, Ganz J, Wenzel I, Brand M. Neural stem cells and neurogenesis in the adult zebrafish brain: Origin, proliferation dynamics, migration and cell fate. Dev. Biol. 2006;295:263\u2013277.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16682018"}]}},{"Citation":"Guo S, Wilson SW, Cooke S, Chitnis AB, Driever W, Rosenthal A. Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons. Dev. Biol. 1999;208:473\u2013487.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10191060"}]}},{"Citation":"Hashimoto H, Yabe T, Hirata T, Shimizu T, Bae Y, Yamanaka Y, Hirano T, Hibi M. Expression of the zinc finger gene fez-like in zebrafish forebrain. Mech. Dev. 2000;97:191\u2013195.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"11025224"}]}},{"Citation":"Hirata T, Nakazawa M, Muraoka O, Nakayama R, Suda Y, Hibi M. Zinc-finger genes Fez and Fez-like function in the establishment of diencephalon subdivisions. Development. 2006;133:3993\u20134004.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16971467"}]}},{"Citation":"Hirata T, Suda Y, Nakao K, Narimatsu M, Hirano T, Hibi M. Zinc finger gene fez-like functions in the formation of subplate neurons and thalamocortical axons. Dev. Dyn. 2004;230:546\u2013556.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15188439"}]}},{"Citation":"Inoue K, Terashima T, Nishikawa T, Takumi T. Fez1 is layer-specifically expressed in the adult mouse neocortex. Eur. J. Neurosci. 2004;20:1\u20138.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15579145"}]}},{"Citation":"Jeong JY, Einhorn Z, Mathur P, Chen L, Lee S, Kawakami K, Guo S. Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl. Development. 2007;134:127\u2013136.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"17164418"}]}},{"Citation":"Kawakami K, Takeda H, Kawakami N, Kobayashi M, Matsuda N, Mishina M. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev. Cell. 2004;7:133\u2013144.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15239961"}]}},{"Citation":"Kokoeva MV, Yin H, Flier JS. Neurogenesis in the hypothalamus of adult mice: potential role in energy balance. Science. 2005;310:679\u2013683.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16254185"}]}},{"Citation":"Kokoeva MV, Yin H, Flier JS. Evidence for constitutive neural cell proliferation in the adult murine hypothalamus. J. Comp. Neurol. 2007;505:209\u2013220.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"17853440"}]}},{"Citation":"Lam CS, Marz M, Strahle U. gfap and nestin reporter lines reveal characteristics of neural progenitors in the adult zebrafish brain. Dev. Dyn. 2009;238:475\u2013486.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"19161226"}]}},{"Citation":"Levkowitz G, Zeller J, Sirotkin HI, French D, Schilbach S, Hashimoto H, Hibi M, Talbot WS, Rosenthal A. Zinc finger protein too few controls the development of monoaminergic neurons. Nat. Neurosci. 2003;6:28\u201333.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"12469125"}]}},{"Citation":"Merkle FT, Tramontin AD, Garcia-Verdugo JM, Alvarez-Buylla A. Radial glia give rise to adult neural stem cells in the subventricular zone. Proc. Natl. Acad. Sci. U. S. A. 2004;101:17528\u201317532.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pmc"},"@text":"PMC536036"},{"@attributes":{"IdType":"pubmed"},"@text":"15574494"}]}},{"Citation":"Molyneaux BJ, Arlotta P, Hirata T, Hibi M, Macklis JD. Fezl is required for the birth and specification of corticospinal motor neurons. Neuron. 2005;47:817\u2013831.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16157277"}]}},{"Citation":"Mueller T, Wullimann MF. BrdU-, neuroD (nrd)-, and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain. Mech. Dev. 2002;117:123\u2013135.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"12204253"}]}},{"Citation":"Patton EE, Zon LI. The art and design of genetic screens: zebrafish. Nat. Rev. Genet. 2001;2:956\u2013966.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"11733748"}]}},{"Citation":"Reimer MM, Sorensen I, Kuscha V, Frank RE, Liu C, Becker CG, Becker T. Motor neuron regeneration in adult zebrafish. J. Neurosci. 2008;28:8510\u20138516.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pmc"},"@text":"PMC6671064"},{"@attributes":{"IdType":"pubmed"},"@text":"18716209"}]}},{"Citation":"Rink E, Guo S. The too few mutant selectively affects subgroups of monoaminergic neurons in the zebrafish forebrain. Neuroscience. 2004;127:147\u2013154.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15219677"}]}},{"Citation":"Shimizu T, Hibi M. Formation and patterning of the forebrain and olfactory system by zinc-finger genes Fezf1 and Fezf2. Dev. Growth Differ. 2009;51:221\u2013231.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"19222525"}]}},{"Citation":"Taupin P, Gage F. Adult neurogenesis and neural stem cells of the central nervous system in mammals. J. Neurosci. Res. 2002;69:745\u2013749.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"12205667"}]}},{"Citation":"Vascotto SG, Beckham Y, Kelly GM. The zebrafish\u2019s swim to fame as an experimental model in biology. Biochem. Cell Biol. 1997;75:479\u2013485.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"9551173"}]}},{"Citation":"Wang TW, Stromberg GP, Whitney JT, Brower NW, Klymkowsky MW, Parent JM. Sox3 expression identifies neural progenitors in persistent neonatal and adult mouse forebrain germinative zones. J. Comp. Neurol. 2006;497:88\u2013100.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"16680766"}]}},{"Citation":"Wullimann MF, Mueller T. Teleostean and mammalian forebrains contrasted: Evidence from genes to behavior. J. Comp. Neurol. 2004;475:143\u2013162.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15211457"}]}},{"Citation":"Wullimann MF, Puelles L. Postembryonic neural proliferation in the zebrafish forebrain and its relationship to prosomeric domains. Anat. Embryol. (Berl) 1999;199:329\u2013348.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10195307"}]}},{"Citation":"Wullimann MF, Rupp B, Reichert H. Neuroanatomy of the Zebrafish Brain. In: Wullimann MF, editor. A Topological Atlas. Birkh\u00e4user Verlag, Basel. 1996. pp. 1\u2013144."},{"Citation":"Zhao C, Deng W, Gage FH. Mechanisms and functional implications of adult neurogenesis. Cell. 2008;132:645\u2013660.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"18295581"}]}},{"Citation":"Zupanc GK. Adult neurogenesis and neuronal regeneration in the brain of teleost fish. J. Physiol. Paris. 2008;102:357\u2013373.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"18984045"}]}},{"Citation":"Zupanc GK, Hinsch K, Gage FH. Proliferation, migration, neuronal differentiation, and long-term survival of new cells in the adult zebrafish brain. J. Comp. Neurol. 2005;488:290\u2013319.","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15952170"}]}}]}]}}}