{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"24318815"},"DateCompleted":{"Year":"2014","Month":"07","Day":"22"},"DateRevised":{"Year":"2013","Month":"12","Day":"09"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"1940-6029"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"1092","PubDate":{"Year":"2014"}},"Title":"Methods in molecular biology (Clifton, N.J.)","ISOAbbreviation":"Methods Mol Biol"},"ArticleTitle":"Real-time PCR quantification of gene expression in embryonic mouse tissue.","Pagination":{"StartPage":"81","EndPage":"94","MedlinePgn":"81-94"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1007\/978-1-60327-292-6_6"}],"Abstract":{"AbstractText":["The Gbx family of transcription factors consists of two closely related proteins GBX1 and GBX2. A defining feature of the GBX family is a highly conserved 60 amino acid DNA-binding domain, which differs by just two amino acids. Gbx1 and Gbx2 are co-expressed in several areas of the developing central nervous system including the forebrain, anterior hindbrain, and spinal cord, suggesting the potential for genetic redundancy. However, there is a spatiotemporal difference in expression of Gbx1 and Gbx2 in the forebrain and spinal cord. Gbx2 has been shown to play a critical role in positioning the midbrain\/hindbrain boundary and developing anterior hindbrain, whereas gene-targeting experiments in mice have revealed an essential function for Gbx1 in the spinal cord for normal locomotion. To determine if Gbx2 could potentially compensate for a loss of Gbx1 in the developing spinal cord, we performed real-time PCR to examine levels of Gbx2 expression in Gbx1(-\/-) spinal cord at embryonic day (E) 13.5, a developmental stage when Gbx2 is rapidly downregulated. We demonstrate that Gbx2 expression is elevated in the spinal cord of Gbx1(-\/-) embryos."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Villalon","ForeName":"Eric","Initials":"E","AffiliationInfo":[{"Affiliation":"Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Schulz","ForeName":"David J","Initials":"DJ"},{"@attributes":{"ValidYN":"Y"},"LastName":"Waters","ForeName":"Samuel T","Initials":"ST"}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013486"},"@text":"Research Support, U.S. Gov't, Non-P.H.S."}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Methods Mol Biol","NlmUniqueID":"9214969","ISSNLinking":"1064-3745"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C490147"},"@text":"Gbx1 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C513182"},"@text":"Gbx2 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D018507","MajorTopicYN":"Y"},"@text":"Gene Expression Regulation, Developmental"}},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"N"},"@text":"Homeodomain Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000096","MajorTopicYN":"Y"},"@text":"biosynthesis"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D051379","MajorTopicYN":"N"},"@text":"Mice"}},{"DescriptorName":{"@attributes":{"UI":"D008967","MajorTopicYN":"N"},"@text":"Molecular Biology"},"QualifierName":[{"@attributes":{"UI":"Q000379","MajorTopicYN":"N"},"@text":"methods"}]},{"DescriptorName":{"@attributes":{"UI":"D016548","MajorTopicYN":"N"},"@text":"Prosencephalon"},"QualifierName":[{"@attributes":{"UI":"Q000196","MajorTopicYN":"N"},"@text":"embryology"}]},{"DescriptorName":{"@attributes":{"UI":"D012249","MajorTopicYN":"N"},"@text":"Rhombencephalon"},"QualifierName":[{"@attributes":{"UI":"Q000196","MajorTopicYN":"N"},"@text":"embryology"}]},{"DescriptorName":{"@attributes":{"UI":"D013116","MajorTopicYN":"N"},"@text":"Spinal Cord"},"QualifierName":[{"@attributes":{"UI":"Q000196","MajorTopicYN":"N"},"@text":"embryology"}]},{"DescriptorName":{"@attributes":{"UI":"D014018","MajorTopicYN":"N"},"@text":"Tissue Distribution"}}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"entrez"},"Year":"2013","Month":"12","Day":"10","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2013","Month":"12","Day":"10","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2014","Month":"7","Day":"23","Hour":"6","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"24318815"},{"@attributes":{"IdType":"doi"},"@text":"10.1007\/978-1-60327-292-6_6"}]}}}}