{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"28848051"},"DateCompleted":{"Year":"2017","Month":"10","Day":"19"},"DateRevised":{"Year":"2021","Month":"12","Day":"04"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"1083-351X"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"292","Issue":"41","PubDate":{"Year":"2017","Month":"Oct","Day":"13"}},"Title":"The Journal of biological chemistry","ISOAbbreviation":"J Biol Chem"},"ArticleTitle":"The transcription factor Gbx2 induces expression of Kruppel-like factor 4 to maintain and induce na\u00efve pluripotency of embryonic stem cells.","Pagination":{"StartPage":"17121","EndPage":"17128","MedlinePgn":"17121-17128"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1074\/jbc.M117.803254"}],"Abstract":{"AbstractText":["The transcription factor Gbx2 (gastrulation brain homeobox 2) is a direct target of the LIF\/STAT3 signaling pathway, maintains mouse embryonic stem cell (mESC) self-renewal, and facilitates mouse epiblast stem cell (mEpiSC) reprogramming to na\u00efve pluripotency. However, the mechanism by which Gbx2 mediates its effects on pluripotency remains unknown. Here, using an RNA-Seq approach, we identified Klf4 (Kruppel-like factor 4) as a direct target of Gbx2. Functional studies indicated that Klf4 mediates the self-renewal-promoting effects of Gbx2, because knockdown of <i>Klf4<\/i> expression abrogated the ability of Gbx2 to maintain the undifferentiated state of mESCs. We also found that Gbx2 largely depends on <i>Klf4<\/i> to reprogram mEpiSCs to a mESC-like state. In summary, our study has uncovered a mechanism by which Gbx2 maintains and induces na\u00efve pluripotency. These findings expand our understanding of the pluripotency control network and may inform the development of culture conditions for improved ESC maintenance and differentiation."],"CopyrightInformation":"\u00a9 2017 by The American Society for Biochemistry and Molecular Biology, Inc."},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Wang","ForeName":"Manman","Initials":"M","AffiliationInfo":[{"Affiliation":"From the Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei 230601, China and."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Tang","ForeName":"Ling","Initials":"L","AffiliationInfo":[{"Affiliation":"From the Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei 230601, China and."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Liu","ForeName":"Dahai","Initials":"D","AffiliationInfo":[{"Affiliation":"From the Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei 230601, China and."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Ying","ForeName":"Qi-Long","Initials":"QL","AffiliationInfo":[{"Affiliation":"the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California 90033."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Ye","ForeName":"Shoudong","Initials":"S","AffiliationInfo":[{"Affiliation":"From the Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei 230601, China and shdye@126.com."}]}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2017","Month":"08","Day":"28"}]},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"J Biol Chem","NlmUniqueID":"2985121R","ISSNLinking":"0021-9258"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C513182"},"@text":"Gbx2 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C000715468"},"@text":"Klf4 protein, mouse"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D000090062"},"@text":"Kruppel-Like Factor 4"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D051741"},"@text":"Kruppel-Like Transcription Factors"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D002460","MajorTopicYN":"N"},"@text":"Cell Line"}},{"DescriptorName":{"@attributes":{"UI":"D065150","MajorTopicYN":"N"},"@text":"Cellular Reprogramming"},"QualifierName":[{"@attributes":{"UI":"Q000502","MajorTopicYN":"Y"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D055785","MajorTopicYN":"N"},"@text":"Gene Knockdown Techniques"}},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"N"},"@text":"Homeodomain Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D000090062","MajorTopicYN":"N"},"@text":"Kruppel-Like Factor 4"}},{"DescriptorName":{"@attributes":{"UI":"D051741","MajorTopicYN":"N"},"@text":"Kruppel-Like Transcription Factors"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D051379","MajorTopicYN":"N"},"@text":"Mice"}},{"DescriptorName":{"@attributes":{"UI":"D000066450","MajorTopicYN":"N"},"@text":"Mouse Embryonic Stem Cells"},"QualifierName":[{"@attributes":{"UI":"Q000166","MajorTopicYN":"N"},"@text":"cytology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]}]},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"Gbx2"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Kruppel-like factor 4 (KLF4)"},{"@attributes":{"MajorTopicYN":"N"},"@text":"cell biology"},{"@attributes":{"MajorTopicYN":"N"},"@text":"differentiation"},{"@attributes":{"MajorTopicYN":"N"},"@text":"embryonic stem cell"},{"@attributes":{"MajorTopicYN":"N"},"@text":"reprogramming"},{"@attributes":{"MajorTopicYN":"N"},"@text":"self-renewal"}]}],"CoiStatement":"The authors declare that they have no conflicts of interest with the contents of this article"},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2017","Month":"6","Day":"20"},{"@attributes":{"PubStatus":"revised"},"Year":"2017","Month":"8","Day":"28"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2017","Month":"8","Day":"30","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2017","Month":"10","Day":"20","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2017","Month":"8","Day":"30","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"2018","Month":"10","Day":"13"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"28848051"},{"@attributes":{"IdType":"pmc"},"@text":"PMC5641878"},{"@attributes":{"IdType":"doi"},"@text":"10.1074\/jbc.M117.803254"},{"@attributes":{"IdType":"pii"},"@text":"S0021-9258(20)33944-2"}]},"ReferenceList":[{"Reference":[{"Citation":"Evans M. 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