{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"29843929"},"DateCompleted":{"Year":"2019","Month":"06","Day":"10"},"DateRevised":{"Year":"2019","Month":"06","Day":"19"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"1879-1476"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"46","Issue":"1","PubDate":{"Year":"2019","Month":"Feb"}},"Title":"Auris, nasus, larynx","ISOAbbreviation":"Auris Nasus Larynx"},"ArticleTitle":"MicroRNA-4497 functions as a tumor suppressor in laryngeal squamous cell carcinoma via negatively modulation the GBX2.","Pagination":{"StartPage":"106","EndPage":"113","MedlinePgn":"106-113"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1016\/j.anl.2018.05.005"},{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"S0385-8146(18)30168-8"}],"Abstract":{"AbstractText":[{"@attributes":{"Label":"OBJECTIVE","NlmCategory":"OBJECTIVE"},"@text":"MicroRNAs (miRNAs) are aberrantly expressed in various tumors and play a critical role in the progression and development of tumors. However, there is little information about the role of miR-4497 in laryngeal squamous cell carcinoma (LSCC). The aim of this study is to investigate the role of miR-4497 in LSCC."},{"@attributes":{"Label":"METHODS","NlmCategory":"METHODS"},"@text":"MiR-4497 expression in tumor tissues and adjacent normal tissues was measured by RT-PCR. The effects of miR-4497 on cell viability and apoptosis were evaluated by the MTT assay, Flow cytometry and caspase-3 activity assay. Western blot analysis was used to measure the expression of various proteins. Bioinformatic analysis and luciferase reporter assay were applied to investigate the relationship between miR-4497 and GBX2."},{"@attributes":{"Label":"RESULTS","NlmCategory":"RESULTS"},"@text":"We found that miR-4497 expression was downregulated in LSCC tumor tissues and cell lines compared to the normal counterparts. Overexpression of miR-4497 inhibits the proliferation and induces apoptosis of LSCC cells accompanied by the down-regulation of anti-apoptotic Bcl-2 proteins. Mechanisms investigation revealed that GBX2 is a direct target of miR-4497. miR-4497 expression was inversely correlated with GBX2 expression in LSCC tissues. Moreover, overexpression of miR-4497 leads to the activation of ERK, JNK but not p38. Inhibition of ERK by specific inhibitor SCH772984 could interfere the apoptosis induced by overexpression of miR-4497."},{"@attributes":{"Label":"CONCLUSION","NlmCategory":"CONCLUSIONS"},"@text":"Therefore, our results indicate that miR-4497 may play a suppressive role in LSCC by targeting GBX2, which offer new insights into the tumorigenesis of LSCC."}],"CopyrightInformation":"Copyright \u00a9 2018 Elsevier B.V. All rights reserved."},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Chen","ForeName":"Xudong","Initials":"X","AffiliationInfo":[{"Affiliation":"Department of Otolaryngology, The First Hospital of Ningbo, Ningbo, People's Republic of China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Zhang","ForeName":"Litao","Initials":"L","AffiliationInfo":[{"Affiliation":"Department of Otolaryngology, The First Hospital of Ningbo, Ningbo, People's Republic of China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Tang","ForeName":"Shixiong","Initials":"S","AffiliationInfo":[{"Affiliation":"Department of Otolaryngology, The First Hospital of Ningbo, Ningbo, People's Republic of China. Electronic address: wizardcxd@163.com."}]}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2018","Month":"05","Day":"26"}]},"MedlineJournalInfo":{"Country":"Netherlands","MedlineTA":"Auris Nasus Larynx","NlmUniqueID":"7708170","ISSNLinking":"0385-8146"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C000595707"},"@text":"BCL2 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C494811"},"@text":"BCL2L1 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C084958"},"@text":"GBX2 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C579095"},"@text":"MCL1 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C000631510"},"@text":"MIRN4497 microRNA, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D035683"},"@text":"MicroRNAs"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D064549"},"@text":"Myeloid Cell Leukemia Sequence 1 Protein"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D019253"},"@text":"Proto-Oncogene Proteins c-bcl-2"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D012333"},"@text":"RNA, Messenger"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D051020"},"@text":"bcl-X Protein"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D017209","MajorTopicYN":"N"},"@text":"Apoptosis"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D015153","MajorTopicYN":"N"},"@text":"Blotting, Western"}},{"DescriptorName":{"@attributes":{"UI":"D045744","MajorTopicYN":"N"},"@text":"Cell Line, Tumor"}},{"DescriptorName":{"@attributes":{"UI":"D049109","MajorTopicYN":"N"},"@text":"Cell Proliferation"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D002470","MajorTopicYN":"N"},"@text":"Cell Survival"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D015169","MajorTopicYN":"N"},"@text":"Colony Count, Microbial"}},{"DescriptorName":{"@attributes":{"UI":"D005434","MajorTopicYN":"N"},"@text":"Flow Cytometry"}},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"N"},"@text":"Homeodomain Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D007822","MajorTopicYN":"N"},"@text":"Laryngeal Neoplasms"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D020935","MajorTopicYN":"N"},"@text":"MAP Kinase Signaling System"}},{"DescriptorName":{"@attributes":{"UI":"D035683","MajorTopicYN":"N"},"@text":"MicroRNAs"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000502","MajorTopicYN":"Y"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D064549","MajorTopicYN":"N"},"@text":"Myeloid Cell Leukemia Sequence 1 Protein"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D019253","MajorTopicYN":"N"},"@text":"Proto-Oncogene Proteins c-bcl-2"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D012333","MajorTopicYN":"N"},"@text":"RNA, Messenger"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D060888","MajorTopicYN":"N"},"@text":"Real-Time Polymerase Chain Reaction"}},{"DescriptorName":{"@attributes":{"UI":"D000077195","MajorTopicYN":"N"},"@text":"Squamous Cell Carcinoma of Head and Neck"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"Y"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D051020","MajorTopicYN":"N"},"@text":"bcl-X Protein"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]}]},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"Apoptosis"},{"@attributes":{"MajorTopicYN":"N"},"@text":"GBX2"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Laryngeal squamous cell carcinoma (LSCC)"},{"@attributes":{"MajorTopicYN":"N"},"@text":"MAPKs"},{"@attributes":{"MajorTopicYN":"N"},"@text":"MiR-4497"}]}]},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2018","Month":"2","Day":"12"},{"@attributes":{"PubStatus":"revised"},"Year":"2018","Month":"4","Day":"8"},{"@attributes":{"PubStatus":"accepted"},"Year":"2018","Month":"5","Day":"8"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2018","Month":"5","Day":"31","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2019","Month":"6","Day":"14","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2018","Month":"5","Day":"31","Hour":"6","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"29843929"},{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.anl.2018.05.005"},{"@attributes":{"IdType":"pii"},"@text":"S0385-8146(18)30168-8"}]}}}}