{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"29887380"},"DateCompleted":{"Year":"2019","Month":"04","Day":"03"},"DateRevised":{"Year":"2025","Month":"05","Day":"30"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"1097-4172"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"174","Issue":"2","PubDate":{"Year":"2018","Month":"Jul","Day":"12"}},"Title":"Cell","ISOAbbreviation":"Cell"},"ArticleTitle":"A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits.","Pagination":{"StartPage":"325","EndPage":"337.e14","MedlinePgn":"325-337.e14"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1016\/j.cell.2018.05.020"},{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"S0092-8674(18)30597-X"}],"Abstract":{"AbstractText":["Multiple proteins act co-operatively in mammalian clathrin-mediated endocytosis (CME) to generate endocytic vesicles from the plasma membrane. The principles controlling the activation and organization of the actin cytoskeleton during mammalian CME are, however, not fully understood. Here, we show that the protein FCHSD2 is a major activator of actin polymerization during CME. FCHSD2 deletion leads to decreased ligand uptake caused by slowed pit maturation. FCHSD2 is recruited to endocytic pits by the scaffold protein intersectin via an unusual SH3-SH3 interaction. Here, its flat F-BAR domain binds to the planar region of the plasma membrane surrounding the developing pit forming an annulus. When bound to the membrane, FCHSD2 activates actin polymerization by a mechanism that combines oligomerization and recruitment of N-WASP to PI(4,5)P<sub>2<\/sub>, thus promoting pit maturation. Our data therefore describe a molecular mechanism for linking spatiotemporally the plasma membrane to a force-generating actin platform guiding endocytic vesicle maturation."],"CopyrightInformation":"Copyright \u00a9 2018 MRC Laboratory of Molecular Biology. Published by Elsevier Inc. All rights reserved."},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Almeida-Souza","ForeName":"Leonardo","Initials":"L","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: lalmeida@mrc-lmb.cam.ac.uk."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Frank","ForeName":"Rene A W","Initials":"RAW","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Garc\u00eda-Nafr\u00eda","ForeName":"Javier","Initials":"J","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Colussi","ForeName":"Adeline","Initials":"A","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Gunawardana","ForeName":"Nushan","Initials":"N","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Johnson","ForeName":"Christopher M","Initials":"CM","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Yu","ForeName":"Minmin","Initials":"M","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Howard","ForeName":"Gillian","Initials":"G","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Andrews","ForeName":"Byron","Initials":"B","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Vallis","ForeName":"Yvonne","Initials":"Y","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"McMahon","ForeName":"Harvey T","Initials":"HT","AffiliationInfo":[{"Affiliation":"MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: hmm@mrc-lmb.cam.ac.uk."}]}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"MC_U105178795","Acronym":"MRC_","Agency":"Medical Research Council","Country":"United Kingdom"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2018","Month":"06","Day":"07"}]},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Cell","NlmUniqueID":"0413066","ISSNLinking":"0092-8674"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D033942"},"@text":"Adaptor Proteins, Vesicular Transport"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D002352"},"@text":"Carrier Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D002966"},"@text":"Clathrin"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C479761"},"@text":"FCHSD2 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C485568"},"@text":"ITSN1 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D008081"},"@text":"Liposomes"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D008565"},"@text":"Membrane Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D034741"},"@text":"RNA, Small Interfering"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D051317"},"@text":"Wiskott-Aldrich Syndrome Protein, Neuronal"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D008841","MajorTopicYN":"N"},"@text":"Actin Cytoskeleton"},"QualifierName":[{"@attributes":{"UI":"Q000502","MajorTopicYN":"Y"},"@text":"physiology"}]},{"DescriptorName":{"@attributes":{"UI":"D033942","MajorTopicYN":"N"},"@text":"Adaptor Proteins, Vesicular Transport"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002352","MajorTopicYN":"N"},"@text":"Carrier Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000037","MajorTopicYN":"N"},"@text":"antagonists & inhibitors"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002462","MajorTopicYN":"N"},"@text":"Cell Membrane"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002966","MajorTopicYN":"N"},"@text":"Clathrin"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D022163","MajorTopicYN":"N"},"@text":"Clathrin-Coated Vesicles"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D004705","MajorTopicYN":"N"},"@text":"Endocytosis"}},{"DescriptorName":{"@attributes":{"UI":"D006367","MajorTopicYN":"N"},"@text":"HeLa Cells"}},{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D008081","MajorTopicYN":"N"},"@text":"Liposomes"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D008565","MajorTopicYN":"N"},"@text":"Membrane Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000037","MajorTopicYN":"N"},"@text":"antagonists & inhibitors"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D008856","MajorTopicYN":"N"},"@text":"Microscopy, Fluorescence"}},{"DescriptorName":{"@attributes":{"UI":"D008958","MajorTopicYN":"N"},"@text":"Models, Molecular"}},{"DescriptorName":{"@attributes":{"UI":"D016297","MajorTopicYN":"N"},"@text":"Mutagenesis, Site-Directed"}},{"DescriptorName":{"@attributes":{"UI":"D034622","MajorTopicYN":"N"},"@text":"RNA Interference"}},{"DescriptorName":{"@attributes":{"UI":"D034741","MajorTopicYN":"N"},"@text":"RNA, Small Interfering"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D051317","MajorTopicYN":"N"},"@text":"Wiskott-Aldrich Syndrome Protein, Neuronal"},"QualifierName":[{"@attributes":{"UI":"Q000737","MajorTopicYN":"N"},"@text":"chemistry"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D018909","MajorTopicYN":"N"},"@text":"src Homology Domains"}}]},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"ARP2\/3"},{"@attributes":{"MajorTopicYN":"N"},"@text":"BAR domain"},{"@attributes":{"MajorTopicYN":"N"},"@text":"FCHSD2"},{"@attributes":{"MajorTopicYN":"N"},"@text":"N-WASP activation"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Nervous Wreck"},{"@attributes":{"MajorTopicYN":"N"},"@text":"actin cytoskeleton"},{"@attributes":{"MajorTopicYN":"N"},"@text":"clathrin-mediated endocytosis"},{"@attributes":{"MajorTopicYN":"N"},"@text":"cytoskeletal forces"},{"@attributes":{"MajorTopicYN":"N"},"@text":"intersectin"},{"@attributes":{"MajorTopicYN":"N"},"@text":"membrane deformation"}]}]},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2017","Month":"5","Day":"7"},{"@attributes":{"PubStatus":"revised"},"Year":"2018","Month":"2","Day":"28"},{"@attributes":{"PubStatus":"accepted"},"Year":"2018","Month":"5","Day":"9"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2018","Month":"6","Day":"12","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2019","Month":"4","Day":"4","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2018","Month":"6","Day":"12","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"2018","Month":"7","Day":"12"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"29887380"},{"@attributes":{"IdType":"pmc"},"@text":"PMC6057269"},{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.cell.2018.05.020"},{"@attributes":{"IdType":"pii"},"@text":"S0092-8674(18)30597-X"}]},"ReferenceList":[{"Reference":[{"Citation":"Battye 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