{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"PubMed-not-MEDLINE","Owner":"NLM"},"PMID":{"@attributes":{"Version":"1"},"@text":"32466118"},"DateRevised":{"Year":"2025","Month":"05","Day":"30"},"Article":{"@attributes":{"PubModel":"Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"2308-3425"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"7","Issue":"2","PubDate":{"Year":"2020","Month":"May","Day":"25"}},"Title":"Journal of cardiovascular development and disease","ISOAbbreviation":"J Cardiovasc Dev Dis"},"ArticleTitle":"<i>Pax9<\/i> and <i>Gbx2<\/i> Interact in the Pharyngeal Endoderm to Control Cardiovascular Development.","ELocationID":[{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"20"},{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.3390\/jcdd7020020"}],"Abstract":{"AbstractText":["The correct formation of the aortic arch arteries depends on a coordinated and regulated gene expression profile within the tissues of the pharyngeal arches. Perturbation of the gene regulatory networks in these tissues results in congenital heart defects affecting the arch arteries and the outflow tract of the heart. Aberrant development of these structures leads to interruption of the aortic arch and double outlet right ventricle, abnormalities that are a leading cause of morbidity in 22q11 Deletion Syndrome (DS) patients. We have recently shown that <i>Pax9<\/i> functionally interacts with the 22q11DS gene <i>Tbx1<\/i> in the pharyngeal endoderm for 4th pharyngeal arch artery morphogenesis, with double heterozygous mice dying at birth with interrupted aortic arch. Mice lacking <i>Pax9<\/i> die perinatally with complex cardiovascular defects and in this study we sought to validate further potential genetic interacting partners of <i>Pax9<\/i>, focussing on <i>Gbx2<\/i> which is down-regulated in the pharyngeal endoderm of <i>Pax9<\/i>-null embryos. Here, we describe the <i>Gbx2-<\/i>null cardiovascular phenotype and demonstrate a genetic interaction between <i>Gbx2<\/i> and <i>Pax9<\/i> in the pharyngeal endoderm during cardiovascular development."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Stothard","ForeName":"Catherine A","Initials":"CA","AffiliationInfo":[{"Affiliation":"Newcastle University Biosciences Institute, Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Mazzotta","ForeName":"Silvia","Initials":"S","AffiliationInfo":[{"Affiliation":"Newcastle University Biosciences Institute, Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Vyas","ForeName":"Arjun","Initials":"A","AffiliationInfo":[{"Affiliation":"Newcastle University Biosciences Institute, Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Schneider","ForeName":"Jurgen E","Initials":"JE","AffiliationInfo":[{"Affiliation":"Biomedical Imaging, University of Leeds, Leeds LS2 9JT, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Mohun","ForeName":"Timothy J","Initials":"TJ","AffiliationInfo":[{"Affiliation":"The Francis Crick Institute, London NW1 1AT, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Henderson","ForeName":"Deborah J","Initials":"DJ","AffiliationInfo":[{"Affiliation":"Newcastle University Biosciences Institute, Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Phillips","ForeName":"Helen M","Initials":"HM","AffiliationInfo":[{"Affiliation":"Newcastle University Biosciences Institute, Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Bamforth","ForeName":"Simon D","Initials":"SD","AffiliationInfo":[{"Affiliation":"Newcastle University Biosciences Institute, Centre for Life, Newcastle-upon-Tyne NE1 3BZ, UK."}]}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"RG\/12\/15\/29935","Acronym":"BHF_","Agency":"British Heart Foundation","Country":"United Kingdom"},{"GrantID":"FS\/16\/8\/31984","Acronym":"BHF_","Agency":"British Heart Foundation","Country":"United Kingdom"},{"GrantID":"RG\/19\/2\/34256","Acronym":"BHF_","Agency":"British Heart Foundation","Country":"United Kingdom"},{"GrantID":"FS\/11\/50\/29038","Acronym":"BHF_","Agency":"British Heart Foundation","Country":"United Kingdom"},{"GrantID":"PG\/16\/39\/32115","Acronym":"BHF_","Agency":"British Heart Foundation","Country":"United Kingdom"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2020","Month":"05","Day":"25"}]},"MedlineJournalInfo":{"Country":"Switzerland","MedlineTA":"J Cardiovasc Dev Dis","NlmUniqueID":"101651414","ISSNLinking":"2308-3425"},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"Gbx2"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Pax9"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Tbx1"},{"@attributes":{"MajorTopicYN":"N"},"@text":"arch arteries"},{"@attributes":{"MajorTopicYN":"N"},"@text":"pharyngeal endoderm"}]}],"CoiStatement":"The authors declare no conflict of interest."},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2020","Month":"3","Day":"25"},{"@attributes":{"PubStatus":"revised"},"Year":"2020","Month":"5","Day":"15"},{"@attributes":{"PubStatus":"accepted"},"Year":"2020","Month":"5","Day":"19"},{"@attributes":{"PubStatus":"entrez"},"Year":"2020","Month":"5","Day":"30","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2020","Month":"5","Day":"30","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2020","Month":"5","Day":"30","Hour":"6","Minute":"1"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"2020","Month":"5","Day":"25"}]},"PublicationStatus":"epublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"32466118"},{"@attributes":{"IdType":"pmc"},"@text":"PMC7344924"},{"@attributes":{"IdType":"doi"},"@text":"10.3390\/jcdd7020020"},{"@attributes":{"IdType":"pii"},"@text":"jcdd7020020"}]},"ReferenceList":[{"Reference":[{"Citation":"Andersen T.A., Troelsen Kde L., Larsen L.A. 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