{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"PubMed-not-MEDLINE","Owner":"NLM"},"PMID":{"@attributes":{"Version":"1"},"@text":"32905879"},"DateRevised":{"Year":"2023","Month":"08","Day":"16"},"Article":{"@attributes":{"PubModel":"Electronic-eCollection"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"2589-0042"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"23","Issue":"9","PubDate":{"Year":"2020","Month":"Sep","Day":"25"}},"Title":"iScience","ISOAbbreviation":"iScience"},"ArticleTitle":"SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells.","Pagination":{"StartPage":"101475","MedlinePgn":"101475"},"ELocationID":[{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"101475"},{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1016\/j.isci.2020.101475"}],"Abstract":{"AbstractText":["Region-specific neural progenitor cells (NPCs) can be generated from human embryonic stem cells (hESCs) by modulating signaling pathways. However, how intrinsic transcriptional factors contribute to the neural regionalization is not well characterized. Here, we generate region-specific NPCs from hESCs and find that SOX1 is highly expressed in NPCs with the rostral hindbrain identity. Moreover, we find that OTX2 inhibits SOX1 expression, displaying exclusive expression between the two factors. Furthermore, <i>SOX1<\/i> knockout (KO) leads to the upregulation of midbrain genes and downregulation of rostral hindbrain genes, indicating that SOX1 is required for specification of rostral hindbrain NPCs. Our SOX1 chromatin immunoprecipitation sequencing analysis reveals that SOX1 binds to the distal region of <i>GBX2<\/i> to activate its expression. Overexpression of <i>GBX2<\/i> largely abrogates <i>SOX1<\/i>-KO-induced aberrant gene expression. Taken together, this study uncovers previously unappreciated role of SOX1 in early neural regionalization and provides new information for the precise control of the OTX2\/GBX2 interface."],"CopyrightInformation":"\u00a9 2020 The Author(s)."},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Liu","ForeName":"Xinyuan","Initials":"X","AffiliationInfo":[{"Affiliation":"CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, CAS Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Fang","ForeName":"Zhuoqing","Initials":"Z","AffiliationInfo":[{"Affiliation":"CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, CAS Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Wen","ForeName":"Jing","Initials":"J","AffiliationInfo":[{"Affiliation":"CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, CAS Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Tang","ForeName":"Fan","Initials":"F","AffiliationInfo":[{"Affiliation":"Department of Histoembryology, Genetics and Developmental Biology, Shanghai Key Laboratory of Reproductive Medicine, Shanghai JiaoTong University School of Medicine, 225 South Chongqing Road, Shanghai 200025, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Liao","ForeName":"Bing","Initials":"B","AffiliationInfo":[{"Affiliation":"Department of Histoembryology, Genetics and Developmental Biology, Shanghai Key Laboratory of Reproductive Medicine, Shanghai JiaoTong University School of Medicine, 225 South Chongqing Road, Shanghai 200025, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Jing","ForeName":"Naihe","Initials":"N","AffiliationInfo":[{"Affiliation":"State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China."},{"Affiliation":"School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai 201210, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Lai","ForeName":"Dongmei","Initials":"D","AffiliationInfo":[{"Affiliation":"International Peace Maternity and Child Health Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200030, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Jin","ForeName":"Ying","Initials":"Y","AffiliationInfo":[{"Affiliation":"CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, CAS Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China."},{"Affiliation":"Department of Histoembryology, Genetics and Developmental Biology, Shanghai Key Laboratory of Reproductive Medicine, Shanghai JiaoTong University School of Medicine, 225 South Chongqing Road, Shanghai 200025, China."},{"Affiliation":"Basic Clinical Research Center, Renji Hospital, Shanghai JiaoTong University School of Medicine, 160 Pujian Road, Shanghai 200127, China."},{"Affiliation":"School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai 201210, China."}]}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2020","Month":"08","Day":"20"}]},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"iScience","NlmUniqueID":"101724038","ISSNLinking":"2589-0042"},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"Developmental Neuroscience"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Molecular Genetics"}]}],"CoiStatement":"The authors declare that they have no conflicts of interest with the contents of this article."},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2019","Month":"12","Day":"10"},{"@attributes":{"PubStatus":"revised"},"Year":"2020","Month":"7","Day":"11"},{"@attributes":{"PubStatus":"accepted"},"Year":"2020","Month":"8","Day":"17"},{"@attributes":{"PubStatus":"medline"},"Year":"2020","Month":"9","Day":"10","Hour":"6","Minute":"1"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2020","Month":"9","Day":"10","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2020","Month":"9","Day":"9","Hour":"20","Minute":"10"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"2020","Month":"8","Day":"20"}]},"PublicationStatus":"epublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"32905879"},{"@attributes":{"IdType":"pmc"},"@text":"PMC7486433"},{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.isci.2020.101475"},{"@attributes":{"IdType":"pii"},"@text":"S2589-0042(20)30667-2"}]},"ReferenceList":[{"Reference":[{"Citation":"Acampora 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