{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"33319795"},"DateCompleted":{"Year":"2021","Month":"05","Day":"20"},"DateRevised":{"Year":"2021","Month":"12","Day":"04"},"Article":{"@attributes":{"PubModel":"Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"2045-2322"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"10","Issue":"1","PubDate":{"Year":"2020","Month":"Dec","Day":"15"}},"Title":"Scientific reports","ISOAbbreviation":"Sci Rep"},"ArticleTitle":"Identification of potential transcription factors that enhance human iPSC generation.","Pagination":{"StartPage":"21950","MedlinePgn":"21950"},"ELocationID":[{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"21950"},{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1038\/s41598-020-78932-9"}],"Abstract":{"AbstractText":["Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platform we have identified and validated the differential gene expression of five transcription factors (TFs) (GBX2, NANOGP8, SP8, PEG3, and ZIC1) that were associated with a remarkable increase in the number of iPSC colonies generated from a patient with Parkinson's disease. We have applied different bioinformatics tools (Gene ontology, protein-protein interaction, and signaling pathways analyses) to investigate the possible roles of these TFs in pluripotency and developmental process. Interestingly, GBX2, NANOGP8, SP8, PEG3, and ZIC1 were found to play a role in maintaining pluripotency, regulating self-renewal stages, and interacting with other factors that are involved in pluripotency regulation including OCT4, SOX2, NANOG, and KLF4. Therefore, the TFs identified in this study could be used as additional transcription factors that enhance reprogramming efficiency to boost iPSC generation technology."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Swaidan","ForeName":"Nuha T","Initials":"NT","AffiliationInfo":[{"Affiliation":"Neurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Salloum-Asfar","ForeName":"Salam","Initials":"S","AffiliationInfo":[{"Affiliation":"Neurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Palangi","ForeName":"Freshteh","Initials":"F","AffiliationInfo":[{"Affiliation":"Neurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Errafii","ForeName":"Khaoula","Initials":"K","AffiliationInfo":[{"Affiliation":"Genomics Core Facility, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation, Doha, Qatar."},{"Affiliation":"College of Health and Life Sciences, Hamad Bin Khalifa University, Education City, Qatar Foundation, Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Soliman","ForeName":"Nada H","Initials":"NH","AffiliationInfo":[{"Affiliation":"Basic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Aboughalia","ForeName":"Ahmed T","Initials":"AT","AffiliationInfo":[{"Affiliation":"Basic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Wali","ForeName":"Abdul Haseeb S","Initials":"AHS","AffiliationInfo":[{"Affiliation":"Basic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Abdulla","ForeName":"Sara A","Initials":"SA","AffiliationInfo":[{"Affiliation":"Neurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation (QF), Doha, Qatar. saabdulla@hbku.edu.qa."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Emara","ForeName":"Mohamed M","Initials":"MM","AffiliationInfo":[{"Affiliation":"Basic Medical Sciences Department, College of Medicine, QU Health, Qatar University, Doha, Qatar. memara@qu.edu.qa."},{"Affiliation":"Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha, Qatar. memara@qu.edu.qa."}]}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2020","Month":"12","Day":"15"}]},"MedlineJournalInfo":{"Country":"England","MedlineTA":"Sci Rep","NlmUniqueID":"101563288","ISSNLinking":"2045-2322"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C000715467"},"@text":"KLF4 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D000090062"},"@text":"Kruppel-Like Factor 4"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D014157"},"@text":"Transcription Factors"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D002454","MajorTopicYN":"N"},"@text":"Cell Differentiation"}},{"DescriptorName":{"@attributes":{"UI":"D049109","MajorTopicYN":"N"},"@text":"Cell Proliferation"}},{"DescriptorName":{"@attributes":{"UI":"D002478","MajorTopicYN":"N"},"@text":"Cells, Cultured"}},{"DescriptorName":{"@attributes":{"UI":"D065150","MajorTopicYN":"N"},"@text":"Cellular Reprogramming"}},{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D057026","MajorTopicYN":"N"},"@text":"Induced Pluripotent Stem Cells"},"QualifierName":[{"@attributes":{"UI":"Q000166","MajorTopicYN":"Y"},"@text":"cytology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D000090062","MajorTopicYN":"N"},"@text":"Kruppel-Like Factor 4"}},{"DescriptorName":{"@attributes":{"UI":"D010300","MajorTopicYN":"N"},"@text":"Parkinson Disease"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"},{"@attributes":{"UI":"Q000473","MajorTopicYN":"N"},"@text":"pathology"}]},{"DescriptorName":{"@attributes":{"UI":"D014157","MajorTopicYN":"N"},"@text":"Transcription Factors"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]}]},"CoiStatement":"The authors declare no competing interests."},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2020","Month":"4","Day":"23"},{"@attributes":{"PubStatus":"accepted"},"Year":"2020","Month":"11","Day":"25"},{"@attributes":{"PubStatus":"entrez"},"Year":"2020","Month":"12","Day":"15","Hour":"8","Minute":"40"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2020","Month":"12","Day":"16","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2021","Month":"5","Day":"21","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"2020","Month":"12","Day":"15"}]},"PublicationStatus":"epublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"33319795"},{"@attributes":{"IdType":"pmc"},"@text":"PMC7738555"},{"@attributes":{"IdType":"doi"},"@text":"10.1038\/s41598-020-78932-9"},{"@attributes":{"IdType":"pii"},"@text":"10.1038\/s41598-020-78932-9"}]},"ReferenceList":[{"Reference":[{"Citation":"Yamanaka S. 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