{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Curated"},"PMID":{"@attributes":{"Version":"1"},"@text":"33550476"},"DateCompleted":{"Year":"2021","Month":"04","Day":"23"},"DateRevised":{"Year":"2022","Month":"05","Day":"31"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"1434-4726"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"278","Issue":"5","PubDate":{"Year":"2021","Month":"May"}},"Title":"European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery","ISOAbbreviation":"Eur Arch Otorhinolaryngol"},"ArticleTitle":"LncRNA FEZF1-AS1 accelerates the migration and invasion of laryngeal squamous cell carcinoma cells through miR-4497 targeting GBX2.","Pagination":{"StartPage":"1523","EndPage":"1535","MedlinePgn":"1523-1535"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1007\/s00405-021-06636-5"}],"Abstract":{"AbstractText":[{"@attributes":{"Label":"BACKGROUND","NlmCategory":"BACKGROUND"},"@text":"MiR-4497 has been previously proved to exert an anti-cancer role in laryngeal squamous cell carcinoma (LSCC) by negatively regulating gastrulation brain homeobox 2 (GBX2). However, the mechanism of miR-4497 in LSCC has yet to be fully elucidated. This study intended to investigate the role of FEZF1-AS1 in the migration and invasion of LSCC cells and clarified its mechanism through miR-4497 and GBX2."},{"@attributes":{"Label":"METHODS","NlmCategory":"METHODS"},"@text":"qPCR evaluated the expression of FEZF1-AS1, miR-4497 and GBX2 in LSCC tissues and cells, compared with controls. Western blotting analyzed GBX2, E-cadherin, N-cadherin and Vimentin. CCK8, wound healing and transwell assays assessed the viability, migration and invasion of TU686 and UM-SCC-17A cells. Luciferase reporter assay affirmed the interplay of miR-4497 with FEZF1-AS1 or GBX2 and Pearson's correlation analysis explored the association between each two genes in both tumor and non-tumor tissues."},{"@attributes":{"Label":"RESULTS","NlmCategory":"RESULTS"},"@text":"FEZF1-AS1 was highly expressed in LSCC tissues and cells. Silence or elevation of FEZF1-AS1 inhibited or promoted the migration and invasion of TU686 and UM-SCC-17A cells. FEZF1-AS1 targeted and negatively modulated miR-4497. Inhibition of miR-4497 markedly restored the FEZF1-AS1 silence-repressed cell viability of TU686 and UM-SCC-17A cells. Further, FEZF1-AS1 could positively regulate GBX2 via negative regulation of miR-4497. In these two cells, GBX2 deficiency reversed the promoting impacts of miR-4497 repression on migration and invasion."},{"@attributes":{"Label":"CONCLUSION","NlmCategory":"CONCLUSIONS"},"@text":"Taken together, FEZF1-AS1, heightened in LSCC tissues and cells, promotes cell migration and invasion of LSCC cells via targeting miR-4497 that inhibits GBX2. The finding may offer new options for the treatment of this cancer."}]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Chen","ForeName":"Xudong","Initials":"X","Identifier":[{"@attributes":{"Source":"ORCID"},"@text":"0000-0002-1673-4696"}],"AffiliationInfo":[{"Affiliation":"Department of Otolaryngology, First Hospital of Ningbo City, Ningbo, Zhejiang, 315000, People's Republic of China. chenxudong_xdc@163.com."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Cheng","ForeName":"Peng","Initials":"P","AffiliationInfo":[{"Affiliation":"Department of Otolaryngology, First Hospital of Ningbo City, Ningbo, Zhejiang, 315000, People's Republic of China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Hu","ForeName":"Cihao","Initials":"C","AffiliationInfo":[{"Affiliation":"Department of Otolaryngology, First Hospital of Ningbo City, Ningbo, Zhejiang, 315000, People's Republic of China."}]}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"2020360873","Agency":"the Medical and Health Project of Zhejiang Province","Country":""}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2021","Month":"02","Day":"07"}]},"MedlineJournalInfo":{"Country":"Germany","MedlineTA":"Eur Arch Otorhinolaryngol","NlmUniqueID":"9002937","ISSNLinking":"0937-4477"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C084958"},"@text":"GBX2 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"C000631510"},"@text":"MIRN4497 microRNA, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D035683"},"@text":"MicroRNAs"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D062085"},"@text":"RNA, Long Noncoding"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D049109","MajorTopicYN":"N"},"@text":"Cell Proliferation"}},{"DescriptorName":{"@attributes":{"UI":"D006258","MajorTopicYN":"Y"},"@text":"Head and Neck Neoplasms"}},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"N"},"@text":"Homeodomain Proteins"}},{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D035683","MajorTopicYN":"Y"},"@text":"MicroRNAs"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D062085","MajorTopicYN":"Y"},"@text":"RNA, Long Noncoding"}},{"DescriptorName":{"@attributes":{"UI":"D000077195","MajorTopicYN":"N"},"@text":"Squamous Cell Carcinoma of Head and Neck"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]}]},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"FEZF1-AS1"},{"@attributes":{"MajorTopicYN":"N"},"@text":"GBX2"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Invasion"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Laryngeal squamous cell carcinoma"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Migration"},{"@attributes":{"MajorTopicYN":"N"},"@text":"miR-4497"}]}]},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2020","Month":"10","Day":"14"},{"@attributes":{"PubStatus":"accepted"},"Year":"2021","Month":"1","Day":"20"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2021","Month":"2","Day":"8","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"medline"},"Year":"2021","Month":"4","Day":"24","Hour":"6","Minute":"0"},{"@attributes":{"PubStatus":"entrez"},"Year":"2021","Month":"2","Day":"7","Hour":"20","Minute":"52"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"33550476"},{"@attributes":{"IdType":"doi"},"@text":"10.1007\/s00405-021-06636-5"},{"@attributes":{"IdType":"pii"},"@text":"10.1007\/s00405-021-06636-5"}]},"ReferenceList":[{"Reference":[{"Citation":"Zhang X, Cavus O, Zhou Y, Dusitkasem S (2018) Airway management during anesthetic induction of secondary laryngectomy for recurrent laryngeal cancer: three cases of report and analysis. Front Med 5:264. https:\/\/doi.org\/10.3389\/fmed.2018.00264","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.3389\/fmed.2018.00264"}]}},{"Citation":"Mosca L, Minopoli M, Pagano M, Vitiello F, Carriero MV, Cacciapuoti G, Porcelli M (2020) Effects of S-adenosyl-L-methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol 56(5):1212\u20131224. https:\/\/doi.org\/10.3892\/ijo.2020.5011","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.3892\/ijo.2020.5011"},{"@attributes":{"IdType":"pubmed"},"@text":"32319579"},{"@attributes":{"IdType":"pmc"},"@text":"7115356"}]}},{"Citation":"Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA 68(6):394\u2013424. https:\/\/doi.org\/10.3322\/caac.21492","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.3322\/caac.21492"},{"@attributes":{"IdType":"pubmed"},"@text":"30207593"}]}},{"Citation":"Steuer CE, El-Deiry M, Parks JR, Higgins KA, Saba NF (2017) An update on larynx cancer. CA 67(1):31\u201350. https:\/\/doi.org\/10.3322\/caac.21386","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.3322\/caac.21386"},{"@attributes":{"IdType":"pubmed"},"@text":"27898173"}]}},{"Citation":"Khan JA, Siddque MA, Haque MN, Kundu SC, Ahmed MU, Bhuiyan AS (2015) Etiology of laryngeal squamous cell carcinoma: study of 50 cases in Mymensingh Medical College Hospital. MMJ 24(3):492\u2013496","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"26329945"}]}},{"Citation":"Escalante P, Barr\u00eda T, Cancino M, Rahal M, Cerpa L, Sandoval C, Molina-Mellico S, Su\u00e1rez M, Mart\u00ednez M, C\u00e1ceres DD, Qui\u00f1ones LA, Varela NM (2020) Genetic polymorphisms as non-modifiable susceptibility factors to laryngeal cancer. Biosci Rep. https:\/\/doi.org\/10.1042\/bsr20191188","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1042\/bsr20191188"},{"@attributes":{"IdType":"pubmed"},"@text":"32338278"},{"@attributes":{"IdType":"pmc"},"@text":"7201556"}]}},{"Citation":"Chu YL (2020) Circ_0067934 correlates with poor prognosis and promotes laryngeal squamous cell cancer progression by sponging miR-1324. Eur Rev Med Pharmacol Sci 24(8):4320\u20134327. https:\/\/doi.org\/10.26355\/eurrev_202004_21013","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.26355\/eurrev_202004_21013"},{"@attributes":{"IdType":"pubmed"},"@text":"32373969"}]}},{"Citation":"Zhao R, Tian L, Zhao B, Sun Y, Cao J, Chen K, Li F, Li M, Shang D, Liu M (2020) FADS1 promotes the progression of laryngeal squamous cell carcinoma through activating AKT\/mTOR signaling. Cell Death Dis 11(4):272. https:\/\/doi.org\/10.1038\/s41419-020-2457-5","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1038\/s41419-020-2457-5"},{"@attributes":{"IdType":"pubmed"},"@text":"32332698"},{"@attributes":{"IdType":"pmc"},"@text":"7181692"}]}},{"Citation":"Cao G, Cui R, Liu C, Zhang Z (2019) MicroRNA regulation of transthyretin in trophoblast biofunction and preeclampsia. Arch Biochem Biophys 676:108129. https:\/\/doi.org\/10.1016\/j.abb.2019.108129","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.abb.2019.108129"},{"@attributes":{"IdType":"pubmed"},"@text":"31593646"}]}},{"Citation":"Cao M, Song F, Yang X, Peng L, Cheng Y, Zheng Q, Liang Y, Wang C (2019) Identification of potential long noncoding RNA biomarker of Mercury compounds in Zebrafish embryos. Chem Res Toxicol 32(5):878\u2013886. https:\/\/doi.org\/10.1021\/acs.chemrestox.9b00029","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1021\/acs.chemrestox.9b00029"},{"@attributes":{"IdType":"pubmed"},"@text":"30912647"}]}},{"Citation":"Liu Y, Yang Y, Li L, Liu Y, Geng P, Li G, Song H (2018) LncRNA SNHG1 enhances cell proliferation, migration, and invasion in cervical cancer. Biochem Cell Biol 96(1):38\u201343. https:\/\/doi.org\/10.1139\/bcb-2017-0188","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1139\/bcb-2017-0188"},{"@attributes":{"IdType":"pubmed"},"@text":"28930646"}]}},{"Citation":"Wei GH, Wang X (2017) lncRNA MEG3 inhibit proliferation and metastasis of gastric cancer via p53 signaling pathway. Eur Rev Med Pharmacol Sci 21(17):3850\u20133856","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"28975980"}]}},{"Citation":"Xu Z, Xi K (2019) LncRNA RGMB-AS1 promotes laryngeal squamous cell carcinoma cells progression via sponging miR-22\/NLRP3 axis. Biomed Pharmacother 118:109222. https:\/\/doi.org\/10.1016\/j.biopha.2019.109222","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.biopha.2019.109222"},{"@attributes":{"IdType":"pubmed"},"@text":"31351424"}]}},{"Citation":"Bian Z, Zhang J, Li M, Feng Y, Wang X, Zhang J, Yao S, Jin G, Du J, Han W, Yin Y, Huang S, Fei B, Zou J, Huang Z (2018) LncRNA-FEZF1-AS1 promotes tumor proliferation and metastasis in colorectal cancer by regulating PKM2 signaling. Clin Cancer Res 24(19):4808\u20134819. https:\/\/doi.org\/10.1158\/1078-0432.ccr-17-2967","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1158\/1078-0432.ccr-17-2967"},{"@attributes":{"IdType":"pubmed"},"@text":"29914894"},{"@attributes":{"IdType":"pmc"},"@text":"29914894"}]}},{"Citation":"Liu YW, Xia R, Lu K, Xie M, Yang F, Sun M, De W, Wang C, Ji G (2017) LincRNAFEZF1-AS1 represses p21 expression to promote gastric cancer proliferation through LSD1-Mediated H3K4me2 demethylation. Mol Cancer 16(1):39. https:\/\/doi.org\/10.1186\/s12943-017-0588-9","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1186\/s12943-017-0588-9"},{"@attributes":{"IdType":"pubmed"},"@text":"28209170"},{"@attributes":{"IdType":"pmc"},"@text":"5314465"}]}},{"Citation":"Zhao X, Cheng Z, Wang J (2018) Long Noncoding RNA FEZF1-AS1 Promotes proliferation and inhibits apoptosis in ovarian cancer by activation of JAK-STAT3 pathway. Med Sci Monit 24:8088\u20138095. https:\/\/doi.org\/10.12659\/msm.911194","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.12659\/msm.911194"},{"@attributes":{"IdType":"pubmed"},"@text":"30416194"},{"@attributes":{"IdType":"pmc"},"@text":"6243867"}]}},{"Citation":"Yang L, Ye Y, Chu J, Jia J, Qu Y, Sun T, Yin H, Ming L, Wan J, He F (2019) Long noncoding RNA FEZF1-AS1 promotes the motility of esophageal squamous cell carcinoma through Wnt\/\u03b2-catenin pathway. Cancer Manag Res 11:4425\u20134435. https:\/\/doi.org\/10.2147\/cmar.s196004","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.2147\/cmar.s196004"},{"@attributes":{"IdType":"pubmed"},"@text":"31191005"},{"@attributes":{"IdType":"pmc"},"@text":"6525003"}]}},{"Citation":"Sun FB, Lin Y, Li SJ, Gao J, Han B, Zhang CS (2018) MiR-210 knockdown promotes the development of pancreatic cancer via upregulating E2F3 expression. Eur Rev Med Pharmacol Sci 22(24):8640\u20138648. https:\/\/doi.org\/10.26355\/eurrev_201812_16628","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.26355\/eurrev_201812_16628"},{"@attributes":{"IdType":"pubmed"},"@text":"30575904"}]}},{"Citation":"Fan Z, Cui H, Xu X, Lin Z, Zhang X, Kang L, Han B, Meng J, Yan Z, Yan X, Jiao S (2015) MiR-125a suppresses tumor growth, invasion and metastasis in cervical cancer by targeting STAT3. Oncotarget 6(28):25266\u201325280. https:\/\/doi.org\/10.18632\/oncotarget.4457","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.18632\/oncotarget.4457"},{"@attributes":{"IdType":"pubmed"},"@text":"26389681"},{"@attributes":{"IdType":"pmc"},"@text":"4694830"}]}},{"Citation":"Chen X, Zhang L, Tang S (2019) MicroRNA-4497 functions as a tumor suppressor in laryngeal squamous cell carcinoma via negatively modulation the GBX2. Auris Nasus Larynx 46(1):106\u2013113. https:\/\/doi.org\/10.1016\/j.anl.2018.05.005","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.anl.2018.05.005"},{"@attributes":{"IdType":"pubmed"},"@text":"29843929"}]}},{"Citation":"Liao Y, Luo H, He Z, Kuang Y, Chen P, Zhang X, Chen J, Wen Q, Xie Y, Ding S (2019) A Combination of UTMD-mediated HIF-1\u03b1 shRNA transfection and TAE in the treatment of hepatic cancer. Biomed Res Int 2019:1937460. https:\/\/doi.org\/10.1155\/2019\/1937460","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1155\/2019\/1937460"},{"@attributes":{"IdType":"pubmed"},"@text":"30911540"},{"@attributes":{"IdType":"pmc"},"@text":"6399560"}]}},{"Citation":"Thomson DW, Dinger ME (2016) Endogenous microRNA sponges: evidence and controversy. Nat Rev Genet 17(5):272\u2013283. https:\/\/doi.org\/10.1038\/nrg.2016.20","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1038\/nrg.2016.20"},{"@attributes":{"IdType":"pubmed"},"@text":"27040487"}]}},{"Citation":"Tay Y, Rinn J, Pandolfi PP (2014) The multilayered complexity of ceRNA crosstalk and competition. Nature 505(7483):344\u2013352. https:\/\/doi.org\/10.1038\/nature12986","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1038\/nature12986"},{"@attributes":{"IdType":"pubmed"},"@text":"4113481"},{"@attributes":{"IdType":"pmc"},"@text":"4113481"}]}},{"Citation":"Han Y, Hu H, Zhou J (2019) Knockdown of LncRNA SNHG7 inhibited epithelial-mesenchymal transition in prostate cancer though miR-324-3p\/WNT2B axis in vitro. Pathol Res Pract 215(10):152537. https:\/\/doi.org\/10.1016\/j.prp.2019.152537","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.prp.2019.152537"},{"@attributes":{"IdType":"pubmed"},"@text":"31324390"}]}},{"Citation":"Li M, Wang Q, Xue F, Wu Y (2019) lncRNA-CYTOR works as an oncogene through the CYTOR\/miR-3679-5p\/MACC1 axis in colorectal cancer. DNA Cell Biol 38(6):572\u2013582. https:\/\/doi.org\/10.1089\/dna.2018.4548","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1089\/dna.2018.4548"},{"@attributes":{"IdType":"pubmed"},"@text":"31144988"}]}},{"Citation":"Fang Y, Yuan Y, Zhang LL, Lu JW, Feng JF, Hu SN (2018) Downregulated GBX2 gene suppresses proliferation, invasion and angiogenesis of breast cancer cells through inhibiting the Wnt\/\u03b2-catenin signaling pathway. Cancer Biomark 23(3):405\u2013418. https:\/\/doi.org\/10.3233\/cbm-181466","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.3233\/cbm-181466"},{"@attributes":{"IdType":"pubmed"},"@text":"30223390"}]}},{"Citation":"Gao AC, Lou W, Isaacs JT (2000) Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene. Clin Cancer Res 6(2):493\u2013497","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"10690529"}]}},{"Citation":"Xia Y, Tang G, Wang C, Zhong J, Chen Y, Hua L, Li Y, Liu H, Zhu B (2020) Functionalized selenium nanoparticles for targeted siRNA delivery silence Derlin1 and promote antitumor efficacy against cervical cancer. Drug Deliv 27(1):15\u201325. https:\/\/doi.org\/10.1080\/10717544.2019.1667452","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"doi"},"@text":"10.1080\/10717544.2019.1667452"},{"@attributes":{"IdType":"pubmed"},"@text":"31830840"}]}}]}]}}}