{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Automated"},"PMID":{"@attributes":{"Version":"1"},"@text":"37315559"},"DateCompleted":{"Year":"2023","Month":"07","Day":"13"},"DateRevised":{"Year":"2023","Month":"07","Day":"18"},"Article":{"@attributes":{"PubModel":"Print-Electronic"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Electronic"},"@text":"1879-0445"},"JournalIssue":{"@attributes":{"CitedMedium":"Internet"},"Volume":"33","Issue":"13","PubDate":{"Year":"2023","Month":"Jul","Day":"10"}},"Title":"Current biology : CB","ISOAbbreviation":"Curr Biol"},"ArticleTitle":"Spatial transcriptomics reveals a cnidarian segment polarity program in Nematostella vectensis.","Pagination":{"StartPage":"2678","EndPage":"2689.e5","MedlinePgn":"2678-2689.e5"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.1016\/j.cub.2023.05.044"},{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"S0960-9822(23)00676-0"}],"Abstract":{"AbstractText":["During early animal evolution, the emergence of axially polarized segments was central to the diversification of complex bilaterian body plans. Nevertheless, precisely how and when segment polarity pathways arose remains obscure. Here, we demonstrate the molecular basis for segment polarization in developing larvae of the sea anemone Nematostella vectensis. Utilizing spatial transcriptomics, we first constructed a 3D gene expression atlas of developing larval segments. Capitalizing on accurate in silico predictions, we identified Lbx and Uncx, conserved homeodomain-containing genes that occupy opposing subsegmental domains under the control of both bone morphogenetic protein (BMP) signaling and the Hox-Gbx cascade. Functionally, Lbx mutagenesis eliminated all molecular evidence of segment polarization at the larval stage and caused an aberrant mirror-symmetric pattern of retractor muscles (RMs) in primary polyps. These results demonstrate the molecular basis for segment polarity in a non-bilaterian animal, suggesting that polarized metameric structures were present in the Cnidaria-Bilateria common ancestor over 600 million years ago."],"CopyrightInformation":"Copyright \u00a9 2023 Elsevier Inc. All rights reserved."},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"He","ForeName":"Shuonan","Initials":"S","AffiliationInfo":[{"Affiliation":"Stowers Institute for Medical Research, Kansas City, MO 64110, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Shao","ForeName":"Wanqing","Initials":"W","AffiliationInfo":[{"Affiliation":"Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA; Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63110, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Chen","ForeName":"Shiyuan Cynthia","Initials":"SC","AffiliationInfo":[{"Affiliation":"Stowers Institute for Medical Research, Kansas City, MO 64110, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Wang","ForeName":"Ting","Initials":"T","AffiliationInfo":[{"Affiliation":"Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA; Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63110, USA; McDonnell Genome Institute, Washington University School of Medicine, St. Louis, MO 63108, USA."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Gibson","ForeName":"Matthew C","Initials":"MC","AffiliationInfo":[{"Affiliation":"Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Department of Anatomy and Cell Biology, The University of Kansas School of Medicine, Kansas City, KS 66160, USA. Electronic address: mg2@stowers.org."}]}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"R01 HG007175","Acronym":"HG","Agency":"NHGRI NIH HHS","Country":"United States"},{"GrantID":"U24 ES026699","Acronym":"ES","Agency":"NIEHS NIH HHS","Country":"United States"},{"GrantID":"U01 CA200060","Acronym":"CA","Agency":"NCI NIH HHS","Country":"United States"},{"GrantID":"U01 HG009391","Acronym":"HG","Agency":"NHGRI NIH HHS","Country":"United States"},{"GrantID":"U41 HG010972","Acronym":"HG","Agency":"NHGRI NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D052061"},"@text":"Research Support, N.I.H., Extramural"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"}]},"ArticleDate":[{"@attributes":{"DateType":"Electronic"},"Year":"2023","Month":"06","Day":"13"}]},"MedlineJournalInfo":{"Country":"England","MedlineTA":"Curr Biol","NlmUniqueID":"9107782","ISSNLinking":"0960-9822"},"CitationSubset":["IM"],"CommentsCorrectionsList":{"CommentsCorrections":[{"@attributes":{"RefType":"UpdateOf"},"RefSource":"bioRxiv. 2023 Jan 10:2023.01.09.523347. doi: 10.1101\/2023.01.09.523347.","PMID":{"@attributes":{"Version":"1"},"@text":"36711919"}},{"@attributes":{"RefType":"CommentIn"},"RefSource":"Curr Biol. 2023 Jul 10;33(13):R717-R719. doi: 10.1016\/j.cub.2023.03.034.","PMID":{"@attributes":{"Version":"1"},"@text":"37433272"}}]},"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D012615","MajorTopicYN":"Y"},"@text":"Sea Anemones"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D059467","MajorTopicYN":"N"},"@text":"Transcriptome"}},{"DescriptorName":{"@attributes":{"UI":"D005801","MajorTopicYN":"N"},"@text":"Genes, Homeobox"}},{"DescriptorName":{"@attributes":{"UI":"D015398","MajorTopicYN":"N"},"@text":"Signal Transduction"}},{"DescriptorName":{"@attributes":{"UI":"D010802","MajorTopicYN":"N"},"@text":"Phylogeny"}}]},"KeywordList":[{"@attributes":{"Owner":"NOTNLM"},"Keyword":[{"@attributes":{"MajorTopicYN":"N"},"@text":"Cnidaria"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Lbx"},{"@attributes":{"MajorTopicYN":"N"},"@text":"Uncx"},{"@attributes":{"MajorTopicYN":"N"},"@text":"germ-layer evolution"},{"@attributes":{"MajorTopicYN":"N"},"@text":"segment polarity"},{"@attributes":{"MajorTopicYN":"N"},"@text":"spatial transcriptomics"}]}],"CoiStatement":"Declaration of interests The authors declare no competing interest."},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2022","Month":"12","Day":"13"},{"@attributes":{"PubStatus":"revised"},"Year":"2023","Month":"4","Day":"16"},{"@attributes":{"PubStatus":"accepted"},"Year":"2023","Month":"5","Day":"18"},{"@attributes":{"PubStatus":"medline"},"Year":"2023","Month":"7","Day":"13","Hour":"6","Minute":"42"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2023","Month":"6","Day":"15","Hour":"1","Minute":"8"},{"@attributes":{"PubStatus":"entrez"},"Year":"2023","Month":"6","Day":"14","Hour":"18","Minute":"41"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"37315559"},{"@attributes":{"IdType":"doi"},"@text":"10.1016\/j.cub.2023.05.044"},{"@attributes":{"IdType":"pii"},"@text":"S0960-9822(23)00676-0"}]}}}}