{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Automated"},"PMID":{"@attributes":{"Version":"1"},"@text":"39832205"},"DateCompleted":{"Year":"2025","Month":"05","Day":"02"},"DateRevised":{"Year":"2026","Month":"01","Day":"27"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0028-2685"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"71","Issue":"6","PubDate":{"Year":"2024","Month":"Dec"}},"Title":"Neoplasma","ISOAbbreviation":"Neoplasma"},"ArticleTitle":"hsa_circ_0021727 facilitates esophageal squamous cell carcinoma progression by stabilizing GBX2 mRNA through interacting with EIF4A3.","Pagination":{"StartPage":"581","EndPage":"593","MedlinePgn":"581-593"},"ELocationID":[{"@attributes":{"EIdType":"doi","ValidYN":"Y"},"@text":"10.4149\/neo_2024_240604N243"},{"@attributes":{"EIdType":"pii","ValidYN":"Y"},"@text":"240604N243"}],"Abstract":{"AbstractText":["Esophageal squamous cell carcinoma (ESCC) has high mortality. The role and regulatory mechanism of hsa_circ_0021727 (circ_0021727) in ESCC remain largely unknown. This study focused on the undiscovered impact of circ_0021727 on cell cycle progression, apoptosis, and angiogenesis of ESCC. We found that circ_0021727 levels were significantly upregulated in ESCC cells. TUNEL, flow cytometry, and tubule formation assay indicated that knockdown of circ_0021727 in ESCC induced cell arrest at the G0\/G1 phase, promoted apoptosis, and inhibited angiogenesis, whereas overexpression of circ_0021727 produced the opposite effect. Gastrulation brain homeobox 2 (GBX2) GBX2 was a downstream target gene of circ_0021727, and overexpression of GBX2 reversed the effect of circ_0021727 knockdown in ESCC progression. The results of the RIP and RNA pull-down showed that circ_0021727 and GBX2 mRNA bound with eukaryotic translation initiation factor 4A3 (EIF4A3). Overexpression of circ_0021727 promoted GBX2 mRNA stability by binding with EIF4A3. In a tumor xenograft model, the knockdown of circ_0021727 inhibited tumor growth, which was reversed by further overexpression of GBX2. In conclusion, circ_0021727 increased GBX2 mRNA stability by recruiting EIF4A3, which promoted cell cycle progression and angiogenesis in ESCC."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Lin","ForeName":"Jie","Initials":"J","AffiliationInfo":[{"Affiliation":"Department of Intensive Medicine\/Comprehensive Intensive Care Unit, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Zhu","ForeName":"Qiuping","Initials":"Q","AffiliationInfo":[{"Affiliation":"Department of Intensive Medicine\/Comprehensive Intensive Care Unit, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China."}]},{"@attributes":{"ValidYN":"Y"},"LastName":"Zeng","ForeName":"Fanlin","Initials":"F","AffiliationInfo":[{"Affiliation":"Department of General Surgery\/Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China."}]}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"}]}},"MedlineJournalInfo":{"Country":"Slovakia","MedlineTA":"Neoplasma","NlmUniqueID":"0377266","ISSNLinking":"0028-2685"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D000079962"},"@text":"RNA, Circular"}},{"RegistryNumber":"EC 3.6.1.-","NameOfSubstance":{"@attributes":{"UI":"C403493"},"@text":"EIF4A3 protein, human"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D018398"},"@text":"Homeodomain Proteins"}},{"RegistryNumber":"EC 2.7.7.-","NameOfSubstance":{"@attributes":{"UI":"D039601"},"@text":"Eukaryotic Initiation Factor-4A"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D012333"},"@text":"RNA, Messenger"}},{"RegistryNumber":"EC 3.6.4.13","NameOfSubstance":{"@attributes":{"UI":"D053487"},"@text":"DEAD-box RNA Helicases"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D000077277","MajorTopicYN":"Y"},"@text":"Esophageal Squamous Cell Carcinoma"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000473","MajorTopicYN":"N"},"@text":"pathology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D000079962","MajorTopicYN":"Y"},"@text":"RNA, Circular"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D004938","MajorTopicYN":"Y"},"@text":"Esophageal Neoplasms"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000473","MajorTopicYN":"N"},"@text":"pathology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D051379","MajorTopicYN":"N"},"@text":"Mice"}},{"DescriptorName":{"@attributes":{"UI":"D018450","MajorTopicYN":"N"},"@text":"Disease Progression"}},{"DescriptorName":{"@attributes":{"UI":"D018398","MajorTopicYN":"Y"},"@text":"Homeodomain Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D039601","MajorTopicYN":"Y"},"@text":"Eukaryotic Initiation Factor-4A"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"},{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D017209","MajorTopicYN":"N"},"@text":"Apoptosis"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D012333","MajorTopicYN":"N"},"@text":"RNA, Messenger"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D015972","MajorTopicYN":"N"},"@text":"Gene Expression Regulation, Neoplastic"}},{"DescriptorName":{"@attributes":{"UI":"D049109","MajorTopicYN":"N"},"@text":"Cell Proliferation"}},{"DescriptorName":{"@attributes":{"UI":"D045744","MajorTopicYN":"N"},"@text":"Cell Line, Tumor"}},{"DescriptorName":{"@attributes":{"UI":"D008819","MajorTopicYN":"N"},"@text":"Mice, Nude"}},{"DescriptorName":{"@attributes":{"UI":"D020871","MajorTopicYN":"N"},"@text":"RNA Stability"}},{"DescriptorName":{"@attributes":{"UI":"D009389","MajorTopicYN":"N"},"@text":"Neovascularization, Pathologic"},"QualifierName":[{"@attributes":{"UI":"Q000235","MajorTopicYN":"N"},"@text":"genetics"}]},{"DescriptorName":{"@attributes":{"UI":"D023041","MajorTopicYN":"N"},"@text":"Xenograft Model Antitumor Assays"}},{"DescriptorName":{"@attributes":{"UI":"D008297","MajorTopicYN":"N"},"@text":"Male"}},{"DescriptorName":{"@attributes":{"UI":"D008807","MajorTopicYN":"N"},"@text":"Mice, Inbred BALB C"}},{"DescriptorName":{"@attributes":{"UI":"D005260","MajorTopicYN":"N"},"@text":"Female"}},{"DescriptorName":{"@attributes":{"UI":"D053487","MajorTopicYN":"N"},"@text":"DEAD-box RNA Helicases"}}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"received"},"Year":"2024","Month":"6","Day":"4"},{"@attributes":{"PubStatus":"accepted"},"Year":"2024","Month":"12","Day":"16"},{"@attributes":{"PubStatus":"medline"},"Year":"2025","Month":"1","Day":"20","Hour":"22","Minute":"56"},{"@attributes":{"PubStatus":"pubmed"},"Year":"2025","Month":"1","Day":"20","Hour":"22","Minute":"55"},{"@attributes":{"PubStatus":"entrez"},"Year":"2025","Month":"1","Day":"20","Hour":"12","Minute":"42"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"39832205"},{"@attributes":{"IdType":"doi"},"@text":"10.4149\/neo_2024_240604N243"},{"@attributes":{"IdType":"pii"},"@text":"240604N243"}]}}}}