{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"6315704"},"DateCompleted":{"Year":"1984","Month":"01","Day":"07"},"DateRevised":{"Year":"2021","Month":"02","Day":"10"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0021-9258"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"258","Issue":"22","PubDate":{"Year":"1983","Month":"Nov","Day":"25"}},"Title":"The Journal of biological chemistry","ISOAbbreviation":"J Biol Chem"},"ArticleTitle":"Characterization of a homogeneous arginyl- and lysyl-tRNA synthetase complex isolated from rat liver. Kinetic mechanism for lysyl-tRNA synthetase.","Pagination":{"StartPage":"13592","EndPage":"13596","MedlinePgn":"13592-6"},"Abstract":{"AbstractText":["Bisubstrate kinetics and end product and dead end inhibition studies were performed on lysyl-tRNA synthetase isolated from rat liver. The kinetic patterns obtained are consistent with a sequential ordered mechanism of substrate addition, tRNA bound first, followed by lysine, and then by ATP. Pyrophosphate and AMP are released in a random fashion with aminoacylated tRNA the last product to dissociate from the enzyme. This is the first report of a kinetic mechanism for lysyl-tRNA synthetase."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Hilderman","ForeName":"R H","Initials":"RH"},{"@attributes":{"ValidYN":"Y"},"LastName":"Zimmerman","ForeName":"J K","Initials":"JK"},{"@attributes":{"ValidYN":"Y"},"LastName":"Dang","ForeName":"C V","Initials":"CV"},{"@attributes":{"ValidYN":"Y"},"LastName":"Grothusen","ForeName":"J R","Initials":"JR"}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"J Biol Chem","NlmUniqueID":"2985121R","ISSNLinking":"0021-9258"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D011756"},"@text":"Diphosphates"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D009097"},"@text":"Multienzyme Complexes"}},{"RegistryNumber":"25125-92-2","NameOfSubstance":{"@attributes":{"UI":"C039763"},"@text":"lysine hydroxamate"}},{"RegistryNumber":"EC 6.1.1.-","NameOfSubstance":{"@attributes":{"UI":"D000604"},"@text":"Amino Acyl-tRNA Synthetases"}},{"RegistryNumber":"EC 6.1.1.19","NameOfSubstance":{"@attributes":{"UI":"D001126"},"@text":"Arginine-tRNA Ligase"}},{"RegistryNumber":"EC 6.1.1.6","NameOfSubstance":{"@attributes":{"UI":"D008250"},"@text":"Lysine-tRNA Ligase"}},{"RegistryNumber":"J41CSQ7QDS","NameOfSubstance":{"@attributes":{"UI":"D015032"},"@text":"Zinc"}},{"RegistryNumber":"K3Z4F929H6","NameOfSubstance":{"@attributes":{"UI":"D008239"},"@text":"Lysine"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000604","MajorTopicYN":"N"},"@text":"Amino Acyl-tRNA Synthetases"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D001126","MajorTopicYN":"N"},"@text":"Arginine-tRNA Ligase"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D011756","MajorTopicYN":"N"},"@text":"Diphosphates"},"QualifierName":[{"@attributes":{"UI":"Q000494","MajorTopicYN":"N"},"@text":"pharmacology"}]},{"DescriptorName":{"@attributes":{"UI":"D007700","MajorTopicYN":"N"},"@text":"Kinetics"}},{"DescriptorName":{"@attributes":{"UI":"D008099","MajorTopicYN":"N"},"@text":"Liver"},"QualifierName":[{"@attributes":{"UI":"Q000201","MajorTopicYN":"Y"},"@text":"enzymology"}]},{"DescriptorName":{"@attributes":{"UI":"D008239","MajorTopicYN":"N"},"@text":"Lysine"},"QualifierName":[{"@attributes":{"UI":"Q000031","MajorTopicYN":"N"},"@text":"analogs & derivatives"},{"@attributes":{"UI":"Q000494","MajorTopicYN":"N"},"@text":"pharmacology"}]},{"DescriptorName":{"@attributes":{"UI":"D008250","MajorTopicYN":"N"},"@text":"Lysine-tRNA Ligase"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D008297","MajorTopicYN":"N"},"@text":"Male"}},{"DescriptorName":{"@attributes":{"UI":"D009097","MajorTopicYN":"N"},"@text":"Multienzyme Complexes"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D051381","MajorTopicYN":"N"},"@text":"Rats"}},{"DescriptorName":{"@attributes":{"UI":"D015032","MajorTopicYN":"N"},"@text":"Zinc"},"QualifierName":[{"@attributes":{"UI":"Q000494","MajorTopicYN":"N"},"@text":"pharmacology"}]}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"1983","Month":"11","Day":"25"},{"@attributes":{"PubStatus":"medline"},"Year":"1983","Month":"11","Day":"25","Hour":"0","Minute":"1"},{"@attributes":{"PubStatus":"entrez"},"Year":"1983","Month":"11","Day":"25","Hour":"0","Minute":"0"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"6315704"},{"@attributes":{"IdType":"pii"},"@text":"S0021-9258(17)43956-1"}]}}}}