{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"6950399"},"DateCompleted":{"Year":"1982","Month":"05","Day":"21"},"DateRevised":{"Year":"2019","Month":"05","Day":"01"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0027-8424"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"78","Issue":"12","PubDate":{"Year":"1981","Month":"Dec"}},"Title":"Proceedings of the National Academy of Sciences of the United States of America","ISOAbbreviation":"Proc Natl Acad Sci U S A"},"ArticleTitle":"Identification of a spectrin-like protein in nonerythroid cells.","Pagination":{"StartPage":"7570","EndPage":"7574","MedlinePgn":"7570-4"},"Abstract":{"AbstractText":["We have demonstrated the existence of a spectrin-like protein in a variety of nonerythroid cultured cells. Indirect immunofluorescence studies with monospecific antispectrin IgG indicated the presence of proteins that have common antigenic determinants to spectrin in embryonic chicken cardiac myocytes, mouse fibroblast lines (3T3, simian virus 4-transformed 3T3), and rat hepatoma lines (HTC, HMOA). Two spectrin-like peptides of 240,000 and 230,000 daltons were immunoprecipitated from octyl glucoside-solubilized embryonic chicken cardiac myocytes, along with associated cytoskeletal proteins. Immunoautoradiographic characterization of the myocyte immunoprecipitate showed that only the spectrin-like 240,000- and 230,000-dalton peptides were stained with monospecific antispectrin IgG and 125I-labeled protein A. One-dimensional partial proteolytic mapping of the myocyte 240,000- and 230,000-dalton peptides showed that these peptides share substantial sequence homology with embryonic chicken erythrocyte spectrin 240,000- and 220,000-dalton peptides."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Goodman","ForeName":"S R","Initials":"SR"},{"@attributes":{"ValidYN":"Y"},"LastName":"Zagon","ForeName":"I S","Initials":"IS"},{"@attributes":{"ValidYN":"Y"},"LastName":"Kulikowski","ForeName":"R R","Initials":"RR"}]},"Language":["eng"],"GrantList":{"@attributes":{"CompleteYN":"Y"},"Grant":[{"GrantID":"HL26059","Acronym":"HL","Agency":"NHLBI NIH HHS","Country":"United States"}]},"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013487"},"@text":"Research Support, U.S. Gov't, P.H.S."}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"Proc Natl Acad Sci U S A","NlmUniqueID":"7505876","ISSNLinking":"0027-8424"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D008565"},"@text":"Membrane Proteins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D010446"},"@text":"Peptide Fragments"}},{"RegistryNumber":"12634-43-4","NameOfSubstance":{"@attributes":{"UI":"D013049"},"@text":"Spectrin"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D002642","MajorTopicYN":"N"},"@text":"Chick Embryo"}},{"DescriptorName":{"@attributes":{"UI":"D003599","MajorTopicYN":"N"},"@text":"Cytoskeleton"},"QualifierName":[{"@attributes":{"UI":"Q000032","MajorTopicYN":"Y"},"@text":"analysis"}]},{"DescriptorName":{"@attributes":{"UI":"D006801","MajorTopicYN":"N"},"@text":"Humans"}},{"DescriptorName":{"@attributes":{"UI":"D008565","MajorTopicYN":"N"},"@text":"Membrane Proteins"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D008970","MajorTopicYN":"N"},"@text":"Molecular Weight"}},{"DescriptorName":{"@attributes":{"UI":"D009206","MajorTopicYN":"N"},"@text":"Myocardium"},"QualifierName":[{"@attributes":{"UI":"Q000648","MajorTopicYN":"N"},"@text":"ultrastructure"}]},{"DescriptorName":{"@attributes":{"UI":"D010446","MajorTopicYN":"N"},"@text":"Peptide Fragments"},"QualifierName":[{"@attributes":{"UI":"Q000032","MajorTopicYN":"N"},"@text":"analysis"}]},{"DescriptorName":{"@attributes":{"UI":"D013049","MajorTopicYN":"N"},"@text":"Spectrin"},"QualifierName":[{"@attributes":{"UI":"Q000032","MajorTopicYN":"N"},"@text":"analysis"},{"@attributes":{"UI":"Q000276","MajorTopicYN":"N"},"@text":"immunology"},{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"1981","Month":"12","Day":"1"},{"@attributes":{"PubStatus":"medline"},"Year":"1981","Month":"12","Day":"1","Hour":"0","Minute":"1"},{"@attributes":{"PubStatus":"entrez"},"Year":"1981","Month":"12","Day":"1","Hour":"0","Minute":"0"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"1982","Month":"6","Day":"1"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"6950399"},{"@attributes":{"IdType":"pmc"},"@text":"PMC349310"},{"@attributes":{"IdType":"doi"},"@text":"10.1073\/pnas.78.12.7570"}]},"ReferenceList":[{"Reference":[{"Citation":"Nature. 1970 Aug 15;227(5259):680-5","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"5432063"}]}},{"Citation":"Biochemistry. 1971 Jun 22;10(13):2606-17","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"4326772"}]}},{"Citation":"J Cell Biol. 1971 Oct;51(1):265-72","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"5000071"}]}},{"Citation":"J Cell Biol. 1974 Jul;62(1):1-19","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"4600883"}]}},{"Citation":"Proc Natl Acad Sci U S A. 1975 Apr;72(4):1359-63","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"1055411"}]}},{"Citation":"J Cell Biol. 1976 Dec;71(3):848-75","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"62755"}]}},{"Citation":"J Biol Chem. 1977 Feb 10;252(3):1102-6","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"320200"}]}},{"Citation":"J Biol Chem. 1977 Apr 25;252(8):2753-63","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"15998"}]}},{"Citation":"Nature. 1977 Mar 10;266(5598):181-3","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"859593"}]}},{"Citation":"J Supramol Struct. 1978;8(4):455-63","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"723278"}]}},{"Citation":"Semin Hematol. 1979 Jan;16(1):21-51","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"370983"}]}},{"Citation":"J Biol Chem. 1979 Apr 10;254(7):2526-32","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"429298"}]}},{"Citation":"J Biol Chem. 1979 Apr 10;254(7):2533-41","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"372182"}]}},{"Citation":"Proc Natl Acad Sci U S A. 1979 May;76(5):2340-4","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"287077"}]}},{"Citation":"Nature. 1979 Oct 18;281(5732):597-9","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"492324"}]}},{"Citation":"Cell. 1980 Dec;22(3):727-38","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"7006832"}]}},{"Citation":"Cell. 1981 Apr;24(1):24-32","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"6453651"}]}}]}]}}}