{"PubmedArticle":{"MedlineCitation":{"@attributes":{"Status":"MEDLINE","Owner":"NLM","IndexingMethod":"Manual"},"PMID":{"@attributes":{"Version":"1"},"@text":"7462324"},"DateCompleted":{"Year":"1981","Month":"04","Day":"24"},"DateRevised":{"Year":"2019","Month":"12","Day":"10"},"Article":{"@attributes":{"PubModel":"Print"},"Journal":{"ISSN":{"@attributes":{"IssnType":"Print"},"@text":"0021-9525"},"JournalIssue":{"@attributes":{"CitedMedium":"Print"},"Volume":"87","Issue":"3 Pt 1","PubDate":{"Year":"1980","Month":"Dec"}},"Title":"The Journal of cell biology","ISOAbbreviation":"J Cell Biol"},"ArticleTitle":"Diphtheria toxin entry into cells is facilitated by low pH.","Pagination":{"StartPage":"828","EndPage":"832","MedlinePgn":"828-32"},"Abstract":{"AbstractText":["At neutral pH, NH4Cl and chloroquine protected cells against diphtheria toxin. A brief exposure of the cells to low pH (4.5-5.5) at 37 degrees completely abolished this protection. When, to cells preincubated with diphtheria toxin and NH4Cl, neutralizing amounts of anti-diphtheria toxin were added before the pH was lowered, the toxic effect was considerably reduced, but it was not completely abolished. A much stronger toxic effect was seen when antibodies were added immediately after incubation at low pH. Upon a short incubation with diphtheria toxin at low pH, the rate of protein synthesis in the cells decreased much faster than when the normal pH was maintained. The data suggest that, at low pH, diphtheria toxin (or its A fragment) penetrates directly through the surface membrane of the cell. The possibility is discussed that, when the medium has a neutral pH, the entry of diphtheria toxin involves adsorptive endocytosis and reduction of the pH in the vesicles possibly by fusion with lysosomes. Low pH did not facilitate the entry of the closely related toxins abrin, ricin, and modeccin."]},"AuthorList":{"@attributes":{"CompleteYN":"Y"},"Author":[{"@attributes":{"ValidYN":"Y"},"LastName":"Sandvig","ForeName":"K","Initials":"K"},{"@attributes":{"ValidYN":"Y"},"LastName":"Olsnes","ForeName":"S","Initials":"S"}]},"Language":["eng"],"PublicationTypeList":{"PublicationType":[{"@attributes":{"UI":"D016428"},"@text":"Journal Article"},{"@attributes":{"UI":"D013485"},"@text":"Research Support, Non-U.S. Gov't"}]}},"MedlineJournalInfo":{"Country":"United States","MedlineTA":"J Cell Biol","NlmUniqueID":"0375356","ISSNLinking":"0021-9525"},"ChemicalList":{"Chemical":[{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D004167"},"@text":"Diphtheria Toxin"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D037102"},"@text":"Lectins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D037121"},"@text":"Plant Lectins"}},{"RegistryNumber":"0","NameOfSubstance":{"@attributes":{"UI":"D054790"},"@text":"Ribosome Inactivating Proteins, Type 2"}},{"RegistryNumber":"01Q9PC255D","NameOfSubstance":{"@attributes":{"UI":"D000643"},"@text":"Ammonium Chloride"}},{"RegistryNumber":"1393-62-0","NameOfSubstance":{"@attributes":{"UI":"D000036"},"@text":"Abrin"}},{"RegistryNumber":"65988-88-7","NameOfSubstance":{"@attributes":{"UI":"C016004"},"@text":"modeccin"}},{"RegistryNumber":"886U3H6UFF","NameOfSubstance":{"@attributes":{"UI":"D002738"},"@text":"Chloroquine"}},{"RegistryNumber":"9009-86-3","NameOfSubstance":{"@attributes":{"UI":"D012276"},"@text":"Ricin"}}]},"CitationSubset":["IM"],"MeshHeadingList":{"MeshHeading":[{"DescriptorName":{"@attributes":{"UI":"D000036","MajorTopicYN":"N"},"@text":"Abrin"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D000643","MajorTopicYN":"N"},"@text":"Ammonium Chloride"},"QualifierName":[{"@attributes":{"UI":"Q000494","MajorTopicYN":"N"},"@text":"pharmacology"}]},{"DescriptorName":{"@attributes":{"UI":"D000818","MajorTopicYN":"N"},"@text":"Animals"}},{"DescriptorName":{"@attributes":{"UI":"D002460","MajorTopicYN":"N"},"@text":"Cell Line"}},{"DescriptorName":{"@attributes":{"UI":"D002462","MajorTopicYN":"N"},"@text":"Cell Membrane"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D002522","MajorTopicYN":"N"},"@text":"Chlorocebus aethiops"}},{"DescriptorName":{"@attributes":{"UI":"D002738","MajorTopicYN":"N"},"@text":"Chloroquine"},"QualifierName":[{"@attributes":{"UI":"Q000494","MajorTopicYN":"N"},"@text":"pharmacology"}]},{"DescriptorName":{"@attributes":{"UI":"D004167","MajorTopicYN":"N"},"@text":"Diphtheria Toxin"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"Y"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D004705","MajorTopicYN":"N"},"@text":"Endocytosis"}},{"DescriptorName":{"@attributes":{"UI":"D006863","MajorTopicYN":"N"},"@text":"Hydrogen-Ion Concentration"}},{"DescriptorName":{"@attributes":{"UI":"D037102","MajorTopicYN":"N"},"@text":"Lectins"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]},{"DescriptorName":{"@attributes":{"UI":"D037121","MajorTopicYN":"Y"},"@text":"Plant Lectins"}},{"DescriptorName":{"@attributes":{"UI":"D054790","MajorTopicYN":"N"},"@text":"Ribosome Inactivating Proteins, Type 2"}},{"DescriptorName":{"@attributes":{"UI":"D012276","MajorTopicYN":"N"},"@text":"Ricin"},"QualifierName":[{"@attributes":{"UI":"Q000378","MajorTopicYN":"N"},"@text":"metabolism"}]}]}},"PubmedData":{"History":{"PubMedPubDate":[{"@attributes":{"PubStatus":"pubmed"},"Year":"1980","Month":"12","Day":"1"},{"@attributes":{"PubStatus":"medline"},"Year":"1980","Month":"12","Day":"1","Hour":"0","Minute":"1"},{"@attributes":{"PubStatus":"entrez"},"Year":"1980","Month":"12","Day":"1","Hour":"0","Minute":"0"},{"@attributes":{"PubStatus":"pmc-release"},"Year":"1981","Month":"6","Day":"1"}]},"PublicationStatus":"ppublish","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"7462324"},{"@attributes":{"IdType":"pmc"},"@text":"PMC2110776"},{"@attributes":{"IdType":"doi"},"@text":"10.1083\/jcb.87.3.828"}]},"ReferenceList":[{"Reference":[{"Citation":"J Bacteriol. 1965 Dec;90(6):1557-62","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"5854584"}]}},{"Citation":"J Bacteriol. 1965 Dec;90(6):1552-6","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"5856535"}]}},{"Citation":"Nat New Biol. 1971 Sep 1;233(35):8-11","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"4999827"}]}},{"Citation":"J Biol Chem. 1973 Jun 10;248(11):3845-50","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"4196585"}]}},{"Citation":"J Cell Biol. 1974 Nov;63(2 Pt 1):430-40","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"4607946"}]}},{"Citation":"Infect Immun. 1975 Apr;11(4):665-74","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"235491"}]}},{"Citation":"Infect Immun. 1975 Apr;11(4):675-84","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"1120609"}]}},{"Citation":"Biochem Biophys Res Commun. 1975 Sep 2;66(1):44-52","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"1164434"}]}},{"Citation":"Exp Cell Res. 1976 Jul;100(2):276-80","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"939253"}]}},{"Citation":"Proc Natl Acad Sci U S A. 1976 Dec;73(12):4449-53","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"63947"}]}},{"Citation":"Biochem Biophys Res Commun. 1977 Apr 11;75(3):699-702","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"193499"}]}},{"Citation":"Proc Natl Acad Sci U S A. 1977 Jul;74(7):2785-9","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"268628"}]}},{"Citation":"Annu Rev Biochem. 1977;46:69-94","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"20040"}]}},{"Citation":"J Biol Chem. 1978 Jul 25;253(14):5069-73","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"97288"}]}},{"Citation":"Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327-31","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"28524"}]}},{"Citation":"J Biol Chem. 1979 Nov 25;254(22):11337-42","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"115867"}]}},{"Citation":"Biochem Biophys Res Commun. 1979 Sep 27;90(2):648-55","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"508328"}]}},{"Citation":"J Biol Chem. 1980 Mar 25;255(6):2247-50","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"6766935"}]}},{"Citation":"J Cell Biol. 1980 Feb;84(2):404-20","ArticleIdList":{"ArticleId":[{"@attributes":{"IdType":"pubmed"},"@text":"6991511"}]}}]}]}}}